Deprenyl rescues dopaminergic neurons in organotypic slice cultures of neonatal rat mesencephalon from N-methyl-d-aspartate toxicity
| Autor: | Akinori Akaike, Hiroshi Katsuki, Seiichiro Shimazu |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
N-Methylaspartate
Tyrosine 3-Monooxygenase Cell Survival Monoamine oxidase Dopamine Excitotoxicity Pharmacology Biology medicine.disease_cause Neuroprotection chemistry.chemical_compound Mesencephalon Culture Techniques Selegiline medicine Animals Rats Wistar Neurotransmitter Neurons Dose-Response Relationship Drug Dopaminergic Stereoisomerism Hydrogen Peroxide Oxidants Immunohistochemistry Rats Neuroprotective Agents Animals Newborn nervous system Biochemistry chemistry Mechanism of action Catecholamine NMDA receptor Dizocilpine Maleate medicine.symptom Excitatory Amino Acid Antagonists medicine.drug |
| Zdroj: | European Journal of Pharmacology. 377:29-34 |
| ISSN: | 0014-2999 |
| Popis: | The potential neuroprotective effect of (−)-deprenyl (R-N,α-dimethyl-N-2-propynylbenzeneethanamine) against N-methyl- d -aspartate (NMDA) excitotoxicity was investigated on rat mesencephalic dopaminergic neurons in organotypic slice cultures. While 24 h application of NMDA (100 μM) caused a marked decrease in the number of surviving dopaminergic neurons, simultaneous application of (−)-deprenyl significantly attenuated the cytotoxic effect of NMDA. (+)-Deprenyl showed a less potent but still significant protective effect against NMDA insult. Pre-treatment of cultures with (−)-deprenyl conferred no protection against subsequent NMDA insult, suggesting that the protective effect of (−)-deprenyl may be independent of its irreversible inhibitory action on monoamine oxidase B. (−)-Deprenyl was also ineffective in preventing cell death induced by H2O2. These results indicated that (−)-deprenyl protects dopaminergic neurons from NMDA excitotoxicity through a mechanism distinct from monoamine oxidase inhibition or detoxification of reaction oxygen species. |
| Databáze: | OpenAIRE |
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