Spectroscopic characterization of Venus at the single molecule level
| Autor: | Gert De Cremer, Johan Hofkens, Peter Dedecker, Jan Michiels, Charlotte C. David, Natalie Verstraeten, Cyrielle Kint |
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| Rok vydání: | 2012 |
| Předmět: |
Yellow fluorescent protein
Time Factors biology Chemistry Analytical chemistry Venus Mass spectrometry biology.organism_classification Fluorescence Characterization (materials science) Molecular Imaging Luminescent Proteins Förster resonance energy transfer Spectrometry Fluorescence Bacterial Proteins Chemical physics biology.protein Escherichia coli Molecule Physical and Theoretical Chemistry Molecular imaging |
| Zdroj: | Photochemicalphotobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology. 11(2) |
| ISSN: | 1474-9092 |
| Popis: | Venus is a recently developed, fast maturating, yellow fluorescent protein that has been used as a probe for in vivo applications. In the present work the photophysical characteristics of Venus were analyzed spectroscopically at the bulk and single molecule level. Through time-resolved single molecule measurements we found that single molecules of Venus display pronounced fluctuations in fluorescence emission, with clear fluorescence on- and off-times. These fluorescence intermittencies were found to occupy a broad range of time scales, ranging from milliseconds to several seconds. Such long off-times can complicate the analysis of single molecule counting experiments or single-molecule FRET experiments. |
| Databáze: | OpenAIRE |
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