Multiple-gene characterization of rotavirus strains: evidence of genetic linkage among the VP7-, VP4-, VP6-, and NSP4-encoding genes
| Autor: | Raquel C. Silva, Fabrício José Benati, Adriana G. Maranhão, Norma Santos, Rodrigo da Silva Lima |
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| Rok vydání: | 2010 |
| Předmět: |
Diarrhea
Rotavirus Genotype Sequence analysis Genetic Linkage Reassortment Reoviridae Viral Nonstructural Proteins medicine.disease_cause Genetic analysis Rotavirus Infections Genetic linkage Virology medicine Cluster Analysis Humans Genotyping Antigens Viral Glycoproteins Toxins Biological Genetics Recombination Genetic biology Reverse Transcriptase Polymerase Chain Reaction Infant Newborn Infant Sequence Analysis DNA biology.organism_classification Infectious Diseases Child Preschool RNA Viral Capsid Proteins Brazil |
| Zdroj: | Journal of medical virology. 82(10) |
| ISSN: | 1096-9071 |
| Popis: | A total of 162 rotavirus strains detected between 1996 and 2006 among individuals with diarrhea in Rio de Janeiro, Brazil, were analyzed by multiple-gene genotyping. Characterization of strains was done by RT-PCR assay for amplification and typing of the VP7-, VP4-, VP6-, and NSP4-encoding genes. Overall, 139 (85.8%) strains belonged to the common group A rotavirus combinations: 67 (41.4%) belonged to genotype G1-P[8]-I1-E1; 18 (11.1%) were G2-P[4]-I2-E2; 11 (6.8%) were G3-P[8]-I1-E1; 12 (7.4%) were G4-P[8]-I1-E1; and 31 (19.1%) were G9-P[8]-I1-E1. Two samples presented mixed genotypes (G1 + G3-P[8]-I1-E1 and G1 + G9-P[9]-I1-E1) and rare combinations, such as G2-P[6]-I2-E2 and G9-P[6]-I2-E2, were detected in six (3.7%) strains. The results suggest a linkage among all four genes. Genotypes G1/G3/G4/G5/G9-P[8] were correlated strongly to I1-E1 genotypes and G2-P[4]/P[6] were correlated to I2-E2 genotypes. Unusual combinations of genes, such as G3-P[9]-I2-E2, G9-P[9]-I1-E2, and G3-P[9]-I3-E3, were observed in 15 (9.3%) strains. The characterization of multiple genes allows a more complete analysis of the rotavirus isolates and provides evidence of natural reassortment of strains. J. Med. Virol. 82:1797–1802, 2010. © 2010 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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