Determination of p-hydroxyphenylacetic acid in cerebrospinal fluid by high-performance liquid chromatography with electrochemical detection
| Autor: | Yayoi Koide, Kiyofumi Kobayashi, Toshikiyo Shohmori |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Adult
Adolescent P-hydroxyphenylacetic acid Metabolite Clinical Biochemistry Electrochemical detection Biochemistry High-performance liquid chromatography Preliminary analysis chemistry.chemical_compound Cerebrospinal fluid Methods Humans Child Chromatography High Pressure Liquid Aged Phenylacetates Chromatography Biochemistry (medical) Mean value Infant Homovanillic Acid General Medicine Middle Aged chemistry Sephadex Child Preschool Chromatography Gel Schizophrenia 3 4-Dihydroxyphenylacetic Acid |
| Zdroj: | Clinica Chimica Acta. 123:161-168 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(82)90125-5 |
| Popis: | Using reversed-phase high-performance liquid chromatography and electrochemical detection with a rapid one-step purification on a Sephadex G-10 column, we have developed a sensitive technique to measure p-hydroxyphenylacetic acid, the oxidatively deaminated metabolite of p-tyramine. The metabolite was measured in cerebrospinal fluid with high recovery and precision. Concentrations of p-hydroxyphenylacetic acid ranged from 4.2 to 17.0 ng/ml with a mean value of 7.8 ng/ml ± 1.1 SEM in lumbar cerebrospinal fluid of 11 control patients. In a preliminary analysis, the concentrations in schizophrenic patients were significantly lower than those in controls. Since p-hydroxyphenylacetic acid in cerebrospinal fluid seems to be derived from the brain, this finding may indicate a decrease in brain formation. The present method should be useful in clinical investigations to clarify a functional role of p-tyramine in the brain. |
| Databáze: | OpenAIRE |
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