Ethylene-Induced Increase in Glutamine Synthetase Activity and mRNA Levels in Hevea brasiliensis Latex Cells
| Autor: | Catherine Perrot-Rechenmann, Jean Guern, J. C. Prevot, J. L. Jacob, V. Pujade-Renaud, A. Clement, H. Chrestin |
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| Rok vydání: | 1994 |
| Předmět: |
Ethylene
Latex Physiology F60 - Physiologie et biochimie végétale Context (language use) Plant Science laticifère Régulation physiologique chemistry.chemical_compound Glutamine synthetase Glutamine synthétase Genetics Protein biosynthesis Métabolisme de l'azote chemistry.chemical_classification biology Réponse de la plante Metabolism biology.organism_classification Hevea brasiliensis Cytosol Enzyme chemistry Biochemistry Activité enzymatique Éthylène Research Article |
| Zdroj: | Plant Physiology |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.105.1.127 |
| Popis: | Ethylene, used as a stimulant of latex production in Hevea brasiliensis, significantly activates the regenerating metabolism within the laticiferous cells. In this context, attention was focused on glutamine synthetase (GS; EC 6.3.1.2), a key enzyme in nitrogen metabolism. A specific and significant activation of the cytosolic glutamine synthetase (GScyt) in the laticiferous cells after ethylene treatment parallels the increase of latex yield. A marked accumulation of the corresponding mRNA was found, but in contrast, a slight and variable increase of the polypeptide level is at the limit of detection by western blotting. The GS response to ethylene might be mediated by ammonia that increases in latex cytosol following ethylene treatment. The physiological significance for such a regulation by ethylene of the GScyt is discussed in terms of the nitrogen requirement for protein synthesis associated with latex regeneration. |
| Databáze: | OpenAIRE |
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