Short-term hyperhomocysteinemia-induced oxidative stress activates retinal glial cells and increases vascular endothelial growth factor expression in rat retina
| Autor: | Namsoo Chang, Insun Lee, Eun Hye Chae, Hwayoung Lee, Ji-Myung Kim, Soo Jung Kim |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A medicine.medical_specialty Hyperhomocysteinemia Time Factors alpha-Tocopherol Vimentin Biology medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Thiobarbituric Acid Reactive Substances Retina Analytical Chemistry Rats Sprague-Dawley chemistry.chemical_compound Random Allocation Folic Acid Internal medicine Glial Fibrillary Acidic Protein medicine Animals Vitamin A Molecular Biology Ganglion cell layer Homocysteine Glial fibrillary acidic protein Superoxide Dismutase Organic Chemistry Retinal General Medicine medicine.disease Scavenger Receptors Class E Immunohistochemistry Rats Vascular endothelial growth factor Oxidative Stress medicine.anatomical_structure Endocrinology chemistry biology.protein Neuroglia Oxidative stress Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 71(5) |
| ISSN: | 0916-8451 |
| Popis: | Hyperhomocysteinemia is associated with an increase in the incidence of vascular diseases, including retinal vascular diseases. We examined the effects of high plasma levels of homocysteine on retinal glial cells and vascular endothelial growth factor (VEGF) expression. Male Sprague-Dawley rats were fed either a 3.0 g/kg homocystine diet or a control diet for 2 week. The homocystine-diet group had higher plasma levels of homocysteine and thiobarbituric acid reactive substances (TBARSs) and lower plasma levels of folate, retinol, alpha-tocopherol, and retinal expression of CuZn superoxide dismutase (SOD) than the controls. The rats fed the homocystine-diet showed an increase in vimentin, glial fibrillary acidic protein (GFAP), and VEGF immunoreactivity in the retina as compared to the controls. The increase in vimentin immunoreactivity in the hyperhomocysteinemic rats was correlated with changes in GFAP immunoreactivity in astrocytes within the ganglion cell layer. We found for the first time that short-term hyperhomocysteinemia-induced oxidative stress activates retinal glial cells and increases VEGF expression in the retina. |
| Databáze: | OpenAIRE |
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