Decellularized Porcine Achilles Tendon Induces Anti-inflammatory Macrophage Phenotype In Vitro and Tendon Repair In Vivo
| Autor: | Ravi Sinha, Henriette Valster, Yuan Huipin, Egemen Deniz Eren, Lorenzo Moroni, Jan de Boer, Jasper Foolen, Aysegul Dede Eren, Sultan Gulce-Iz |
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| Přispěvatelé: | RS: MERLN - Complex Tissue Regeneration (CTR), CTR, Biointerface Science, ICMS Core, EAISI Health |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
musculoskeletal diseases Pathology medicine.medical_specialty Achilles tendon Decellularization business.industry 0206 medical engineering 02 engineering and technology musculoskeletal system 020601 biomedical engineering Regenerative medicine In vitro 3. Good health Tendon 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Tissue engineering In vivo medicine Stem cell business |
| Zdroj: | Journal of Immunology and Regenerative Medicine, 8:100027. Elsevier Journal of Immunology and Regenerative Medicine |
| ISSN: | 2468-4988 |
| Popis: | Decellularized tissues and organs from animal sources are widely used in regenerative medicine and tissue engineering. However, in tendon tissue engineering there is limitation not only in terms of tissue source -allografts and autografts- but also standardization of decellularization techniques. The goal of this study is to decellularize porcine Achilles tendon to be used as an off-the-shelf product for tendon reconstruction. We describe a novel, mild decellularization strategy which retains the biochemical and biomechanical characteristics of native tendon upon decellularization. We further show that decellularized tendon sections in vitro induce tenogenic differentiation in stem cells and anti-inflammatory response in naive macrophages. Upon implantation in an Achilles tendon defectin rabbits, we observed that decellularized tendons integrated with the host tissue without signs of tissue rejection or encapsulation. Together, we demonstrate that decellularized tendons produced with our new protocol bear a great potential for tendon tissue regeneration. |
| Databáze: | OpenAIRE |
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