Topological mapping of 13 epitopes on a subunit of Androctonus australis hemocyanin
| Autor: | Nicolas Boisset, Philippe Billiald, Jean Lamp, Geneviève Motta, Josette Lamp, Jean-Christophe Taveau |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Protein Conformation
medicine.drug_class Protein subunit Immunoelectron microscopy medicine.medical_treatment Androctonus australis Mice Inbred Strains Monoclonal antibody Peptide Mapping Epitope Cell Line Epitopes Mice Structural Biology Buthidae Arachnida Image Processing Computer-Assisted medicine Animals Polyacrylamide gel electrophoresis biology Antibodies Monoclonal Hemocyanin biology.organism_classification Molecular biology Hemocyanins Immunology |
| Zdroj: | Journal of Structural Biology. 103:64-74 |
| ISSN: | 1047-8477 |
| DOI: | 10.1016/1047-8477(90)90087-s |
| Popis: | A topological localization of epitopes on the surface of the Aa6 subunit of Androctonus australis hemocyanin has been carried out. First, immunocomplex strings composed of of native hemocyanin and monoclonal antibodies were examined in the electron microscope and submitted to an image processing by correspondence analysis. The average images were then compared to a three-dimensional model of the 24-mer suggesting that 11 of the 13 epitopes are located in three zones of the subunit surface. Second, the overlaps between the epitopes were then studied by polyacrylamide gel electrophoresis, competitive binding inhibition, and immunoelectron microscopy. Four groups of epitopes were identified. One group was capable of binding exclusively to the free subunit. The other three groups were identical to those found in immunoelectron microscopy. The data are consistent with the existence of a small number of immunodominant regions on the surface of the Aa6 subunit. |
| Databáze: | OpenAIRE |
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