The use of restriction fragment polymorphisms to identify the cell line MCF-7
| Autor: | Jeffrey M. Trent, C. Kent Osborne, Ronald N. Buick, Charles M. McGrath, Kathryn A. Graham, Mark D. Minden |
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| Rok vydání: | 1986 |
| Předmět: |
Genetics
Cancer Research Polymorphism Genetic Breast Neoplasms Biology Cell Line Restriction fragment chemistry.chemical_compound Oncology chemistry MCF-7 Genetic marker Cell culture Polymorphism (computer science) Karyotyping Gene duplication biology.protein Humans Restriction fragment length polymorphism skin and connective tissue diseases Cells Cultured DNA |
| Zdroj: | Breast Cancer Research and Treatment. 8:29-34 |
| ISSN: | 1573-7217 0167-6806 |
| Popis: | We have recently documented variations in the level of N-ras gene amplification in independently passaged MCF-7 cells. To establish if these differences are due to a lack of genetic identity between current MCF-7 passages and the original cell line, we have performed karyological and restriction fragment length polymorphism (RFLP) analyses. Documentation of a combination of restriction fragment patterns which are characteristic of MCF-7 has been facilitated by an analysis of DNA from an early-passage of MCF-7 from the Michigan Cancer Foundation (MCF). In addition, such early passage cells and other MCF-7 lines also share a unique amplification of the N-ras oncogene. Using these criteria and karyotypic analysis it can be shown that a sample of MCF-7 obtained from the American Type Culture Collection (-ATCC) was derived from a different individual than was the original MCF-7 cell line. It is important that researchers verify the relationship of current cell lines to the original MCF-7. Furthermore, the techniques described here provide a powerful tool which may be used to assess the identity of cell stocks. |
| Databáze: | OpenAIRE |
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