Go protein subunit Goα and the secretory process of the natriuretic peptide hormones ANF and BNP
| Autor: | Adolfo J. de Bold, Qiuji Li, Michael Gollob, Asna Choudhry, Tina Georgalis, Cassandra Roeske, Amy Martinuk, Geoffrey N. Hendy |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Gene isoform medicine.medical_specialty Mice 129 Strain G protein Immunoprecipitation Protein subunit Immunocytochemistry Gene Expression GTP-Binding Protein alpha Subunits Gi-Go Endocrinology Internal medicine Natriuretic Peptide Brain medicine Animals cardiovascular diseases Molecular Biology Mice Knockout biology Chemistry Myocardium Chromogranin A Mice Inbred C57BL Protein Transport cardiovascular system biology.protein Atrial Natriuretic Factor hormones hormone substitutes and hormone antagonists Intracellular Hormone |
| Zdroj: | Journal of Molecular Endocrinology. 54:277-288 |
| ISSN: | 1479-6813 0952-5041 |
| DOI: | 10.1530/jme-15-0081 |
| Popis: | Expression of the G protein subunit Goα has been shown to be prominent in the atria of the rat heart and to be significantly associated with atrial natriuretic factor (ANF)-containing atrial-specific secretory granules by immunocytochemistry. In addition, differential expression profile analysis using oligonucleotide arrays has shown that the Goα isoform 1 (Goα1) is 2.3-fold more abundant in the atria than it is in the ventricles. In the present report, we show protein–protein interaction between Goα and ANF by yeast two-hybrid and by immunoprecipitation. A cardiac conditional Goα knockout model developed for the present study showed a 90% decrease in Goα expression and decreased atrial expression and ANF and brain natriuretic peptides (BNP) content. Expression of chromogranin A, a specific atrial granule core constituent, was not affected. Morphometric assessment of atrial tissue showed a very significant decrease in atrial-specific granule density as well as granule core electron density. Atrial electrical activity was not affected. The results obtained are compatible with the suggestion that Goα plays a role in ANF sorting during intracellular vectorial transport and with the presence of a mechanism that preserves the molar relationship between cellular ANF and BNP stores in the face of the decreased production of these hormones. |
| Databáze: | OpenAIRE |
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