An Engineered Multiphase Three-Dimensional Microenvironment to Ensure the Controlled Delivery of Cyclic Strain and Human Growth Differentiation Factor 5 for the Tenogenic Commitment of Human Bone Marrow Mesenchymal Stem Cells
| Autor: | Roberta Campardelli, Claudio Muscari, Francesca Bonafè, Emanuele Giordano, Anna C. Berardi, Carlo Guarnieri, Giovanna Della Porta, Nicola Maffulli, Marco Govoni, Ernesto Reverchon |
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| Přispěvatelé: | Govoni, Marco, Berardi, Anna Concetta, Muscari, Claudio, Campardelli, Roberta, Bonafè, Francesca, Guarnieri, Carlo, Reverchon, Ernesto, Giordano, Emanuele, Maffulli, Nicola, Della Porta, Giovanna |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
bioactive 3D scaffold cyclic strain Biomedical Engineering human growth differentiation factor 5 Clinical uses of mesenchymal stem cells PLGA microcarrier Bioengineering Biochemistry Biomaterials 03 medical and health sciences 0302 clinical medicine Tissue engineering medicine Surgical repair Chemistry Mesenchymal stem cell Growth differentiation factor Microcarrier human bone marrow mesenchymal stem cell Tendon Cell biology 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Stem cell Biomedical engineering |
| Zdroj: | Tissue Engineering Part A. 23:811-822 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2016.0407 |
| Popis: | At present, injuries or rupture of tendons are treated by surgical repair or conservative approaches with unpredictable clinical outcome. Alternative strategies to repair tendon defects without the undesirable side effects associated with the current options are needed. With this in mind, a tissue engineering approach has gained considerable attention as a promising strategy. Here we investigated a synthetic three-dimensional (3D) microenvironment able to interact with stem cells and inducing, via coupled biochemical and physical signals, their early commitment toward the tenogenic lineage. This multiphase 3D construct consisted of a braided hyaluronate elastic band merged with human bone marrow mesenchymal stem cells (hBMSCs) and poly-lactic-co-glycolic acid microcarriers loaded with human growth differentiation factor 5 (hGDF-5) by means of fibrin hydrogel. The multiphase structure allowed hBMSC culture under cyclic strain within a microenvironment where a controlled amount of hGDF-5 was regularly delivered. The cooperative biochemical and physical stimuli induced significantly increased expression of tenogenic markers, such as collagen type I and III, decorin, scleraxis, and tenascin-C, within only 3 days of dynamic hBMSC culture. This approach opens exciting perspectives for future development of engineered tendon tissue substitutes. |
| Databáze: | OpenAIRE |
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