| Popis: |
Milk is an important source of all basic dietary supplements and nutrients. Present investigational study was carried out to evaluate physiochemical and microbiological analysis on total six pasteurized milk brands marketed in Karachi, Pakistan, Pharmacopoeial and reported standard methods was used for every analysis, Physicochemical parameters evaluated includes pH determination, Viscosity, Ash content, Fat contents, calcium and magnesium contents. On organoleptic Evaluation Sample 1,3,4,5 gives sweet taste whereas Sample 2 gives unpleasant and bitter taste whereas sample 6 gives metallic taste. Similarly appearance of Sample 2 and 3 showed white and good flows while Sample 4 and 6 showed White colour, whereas sample 5 showed offwhitecolour. Moisture content found that Sample 1 contains the least amount of moisture content (81.76%) as compared to all the other samples while Sample 5 has the highest moisture content (86.52%). In Ash Content Sample 4 has the highest ash content (0.66%). While Sample 1 has the lowest ash content among all (0.41%). Sample 4 might be rich in other minerals hence the highest ash content (0.66%). The Fat Content showed Sample 5 has the highest fat content (5.4%) while Sample 1 has the lowest (3.2%). Calcium and Magnesium ion (mg/lit) was being carried which showed that Sample 1 has the highest calcium content (765mg/lit) while Sample 3 has the lowest (456mg/lit), it’s surprising for Sample 1 as it had the labeled manufactured date of 2016 and yet packs the highest level of Calcium after a period of time whereas sample 3 has more magnesium than other samples (120mg/lit). Microbial Assay test has been performed to confirm any microbial, presence of E.coli by Most Probable Number (MPN) Method under the method of European Pharmacopoeia in which Sample 6 was the only sample that has E.coli. Then further observation was done on total Aerobic Microbial Count (TAMC) and total Yeast Microbial Count (TYMC), where neither of the samples showed Total yeast mold count (TYMC) but in the case of total aerobic viable count (TAMC) Sample 1 was found to be Nil while Sample 2 gave 4 counts, Sample 3 have total of three counts, Sample 4 gives 2 counts, Sample 5 gives 7 counts and Sample 6 gives 9 aerobic counts. Hence according to the proposed plan all the identification is being done but further evaluation is being needed for the presence of E.coli in Sample 6 for the development and betterment of population and trial methods should be done to remove such products from market for human health and safety. |
