Serum-free isolation and culture system to enhance the proliferation and bone regeneration of adipose tissue-derived mesenchymal stem cells
| Autor: | Sunil C. Kaul, Kazutoshi Sato, Renu Wadhwa, Fujio Sato, Yukiko Kitamura, Osamu Ohneda, Takehiro Itoh, Toshiki Kato |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Bone Regeneration Cell Culture Techniques Cell- and Tissue-Based Therapy Clinical uses of mesenchymal stem cells Enzyme-Linked Immunosorbent Assay Biology Culture Media Serum-Free medicine Humans Bone regeneration Stem cell transplantation for articular cartilage repair Cell Proliferation DNA Primers Analysis of Variance Reverse Transcriptase Polymerase Chain Reaction Mesenchymal stem cell Osteoblast Cell Differentiation Mesenchymal Stem Cells Cell Biology General Medicine Cell biology medicine.anatomical_structure Adipose Tissue Cell culture Karyotyping Immunology Cytokines Female Stem cell Fetal bovine serum Developmental Biology |
| Zdroj: | In vitro cellulardevelopmental biology. Animal. 51(5) |
| ISSN: | 1543-706X |
| Popis: | Cell therapy using human mesenchymal stem cells (MSCs) is an attractive approach for many refractory diseases. Adipose tissue-derived mesenchymal stem cells (AT-MSCs) are considered as a favorable tool due to its abundance in the body, easy proliferation, and high cytokine production potency. In order to avoid the risks associated with the use of fetal bovine serum (FBS) in culture that includes batch variations and contamination with pathogens, development of serum-free culture system has been initiated. We have formulated a completely serum-free culture medium (SFM) that could be used not only for the expansion of AT-MSCs but also for initial isolation. We demonstrate that the AT-MSCs isolated and cultured in serum-free medium (AT-MSCs/SFM) possess high proliferation capacity and differentiation potency to osteoblast, adipocyte, and chondrocyte lineages in vitro. In in vivo bone fraction model analysis, AT-MSCs/SFM showed higher bone repair potency and quality of the regenerated bone than the cells cultured in serum-containing medium (AT-MSCs/SCM). This was attributed to the (i) presence of translated cells in the bone, as evidenced by in vivo imaging of the illuminated translated cells and (ii) high level of expression and induction capacity of AT-MSCs/SFM for cytokine BMP2, CCL2, and CCL5. Taken together, we report a new serum-free culture system for AT-MSCs that is suitable for cell therapy. |
| Databáze: | OpenAIRE |
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