Comparison of the effects of anaerobic and micro-aerophilic incubation on resistance of Helicobacter pylori to metronidazole
| Autor: | DS Rampton, Y. M. Abdi, J. M. Hardie, K.A. Young, R. A. Feldman, N. Banatvala |
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| Rok vydání: | 1999 |
| Předmět: |
Microbiology (medical)
medicine.drug_class Spirillaceae Antibiotics Microbial Sensitivity Tests Biology Microbiology Metronidazole medicine Humans Anaerobiosis Incubation Antibacterial agent Observer Variation Helicobacter pylori Stomach Drug Resistance Microbial General Medicine biology.organism_classification Aerobiosis Anti-Bacterial Agents Anaerobic exercise Bacteria medicine.drug |
| Zdroj: | Journal of medical microbiology. 48(4) |
| ISSN: | 0022-2615 |
| Popis: | To assess the influence of incubation conditions on the resistance of Helicobacter pylori this study compared the effect of micro-aerophilic and anaerobic incubation followed by micro-aerophilic incubation on the measurement of metronidazole resistance of 102 H. pylori isolates, by both disk diffusion and Epsilometer (E)-tests. Anaerobic incubation for 24 h before micro-aerophilic incubation for 48 h consistently increased metronidazole activity in both assay methods. Although statistically significant, this was microbiologically less significant, as only 4 of 102 isolates gave discrepant readings (all four were resistant in micro-aerophilic conditions but susceptible in anaerobic/micro-aerophilic conditions). In all four cases variation was by a few millimeters in zone size (i.e., all were close to the cut-off point). There was 100% agreement between disk diffusion and E-test results. Of 104 observations (52 duplicate assays: 13 strains, two atmospheric conditions, two methods of determining resistance) there was 100% intra-observer and inter-observer agreement with regard to susceptibility and resistance status for both E-test and disk diffusion methods. Anaerobic incubation followed by micro-aerophilic incubation had little effect on the estimation of prevalence of metronidazole resistance and seemed to add little, if any, significant advantage over micro-aerophilic incubation alone. |
| Databáze: | OpenAIRE |
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