Obesity in Midlife Hampers Resting and Sensory‐Evoked Cerebral Blood Flow in Mice

Autor: Hirac Gurden, Jean-Sébastien Silvestre, Christophe Magnan, Paolo Giacobini, Frédéric Pain, Haleh Soleimanzad, Mathilde Lemitre, Mireia Montaner, Nadim Kassis, Gaetan Ternier
Přispěvatelé: Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Lille Neurosciences & Cognition - U 1172 (LilNCog), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Paris-Centre de Recherche Cardiovasculaire (PARCC (UMR_S 970/ U970)), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Laboratoire Charles Fabry / Biophotonique, Laboratoire Charles Fabry (LCF), Institut d'Optique Graduate School (IOGS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut d'Optique Graduate School (IOGS), Gurden, Hirac, ANR-16-CE14-0026,Fat4Brain,Le métabolisme des lipides dans le cerveau est un regulateur essentiel de l'homéostasie énergétique(2016), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Lille Neurosciences & Cognition - U 1172 (LilNCog (ex-JPARC)), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP)
Rok vydání: 2020
Předmět:
Male
Cardiac function curve
Aging
medicine.medical_specialty
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Endocrinology
Diabetes and Metabolism
Mice
Obese
Medicine (miscellaneous)
030209 endocrinology & metabolism
Sensory system
Diet
High-Fat
Mice
03 medical and health sciences
0302 clinical medicine
Endocrinology
Insulin resistance
Internal medicine
Glucose Intolerance
medicine
Animals
Obesity
030212 general & internal medicine
Nutrition and Dietetics
Ejection fraction
business.industry
[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Insulin sensitivity
medicine.disease
Olfactory Bulb
Olfactory bulb
Mice
Inbred C57BL
Smell
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition
[SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging
Cerebral blood flow
Cerebrovascular Circulation
Odorants
Insulin Resistance
business
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Zdroj: Obesity
Obesity, 2020, 29 (1), pp.150-158. ⟨10.1002/oby.23051⟩
Obesity, Wiley, 2020, 29 (1), pp.150-158. ⟨10.1002/oby.23051⟩
ISSN: 1930-739X
1930-7381
DOI: 10.1002/oby.23051
Popis: International audience; Objective : This study aimed to investigate the effects of a high-fat diet (HFD) and aging on resting and activity-dependent cerebral blood flow (CBF).Methods: To run a comparison between obese and age-matched control animals, 6-week-old mice were fed either with regular chow or an HFD for 3 months or 8 months. Glucose tolerance and insulin sensitivity were assessed for metabolic phenotyping. Resting and odor-evoked CBF at the microvascular scale in the olfactory bulb (OB) was investigated by multiexposure speckle imaging. Immunolabeling-enabled imaging of solvent-cleared organs was used to analyze vascular density. The ejection fraction was studied by using cardioechography. Olfactory sensitivity was tested by using a buried-food test.Results: Glucose intolerance and compromised odor-evoked CBF were observed in obese mice in the younger group. Prolonged HFD feeding triggered insulin resistance and stronger impairment in activity-dependent CBF. Aging had a specific negative impact on resting CBF. There was no decrease in vascular density in the OB of obese mice, although cardiac function was impaired at both ages. In addition, decreased olfactory sensitivity was observed only in the older, middle-aged obese mice.Conclusions : OB microvasculature in obese mice showed a specific functional feature characterized by impaired sensory-evoked CBF and a specific deleterious effect of aging on resting CBF.
Databáze: OpenAIRE
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