Isolation and structural analyses of positional isomers of 61,6n-di-O-(N-acetyl-β-d-glucosaminyl)cyclomaltoheptaose (n=2, 3, and 4) and 6-O-[6-O-(N-acetyl-β-d-glucosaminyl)-N-acetyl-β-d-glucosaminyl]cyclomaltoheptaose

Autor: Yasuyo Okada, Sumio Kitahata, Kenichi Hamayasu, Koki Fujita, Masanori Semma, Hitoshi Hashimoto, Kyoko Koizumi, Yoshio Ito
Rok vydání: 2001
Předmět:
Zdroj: Carbohydrate Research. 336:203-211
ISSN: 0008-6215
DOI: 10.1016/s0008-6215(01)00229-4
Popis: 6- O -[6- O -( N -acetyl-β- d -glucosaminyl)- N -acetyl-β- d- glucosaminyl]cyclomaltoheptaose (βCD) and three positional isomers of 6 1 ,6 n -di- O -( N -acetyl-β- d -glucosaminyl)cyclomaltoheptaose ( n =2, 3, and 4) in a mixture of products from βCD and N -acetylglucosamine by the reversed reaction of β- N -acetylhexosaminidase from jack bean were isolated and purified by HPLC. The structures of four isomers of di- N -acetylglucosaminyl-βCDs were determined by FABMS and NMR spectroscopy. The degree of polymerization of the branched oligosaccharides produced by enzymatic degradation with bacterial saccharifying α-amylase (BSA) was established by LC–MS methods.
Databáze: OpenAIRE
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