| Autor: |
Dawn E. Quelle, Michael D. Henry, Steven R. Grossman, J. Nathan Davis, Shari Meyers, David K. Meyerholz, Joseph J. Cullen, Kokou D. Zamba, Songhai Chen, Xiaoyun Tang, Xuefeng Zhang, Seema Paliwal, J. Matthew Barnes, Viviane Palhares Muniz |
| Rok vydání: |
2023 |
| DOI: |
10.1158/1541-7786.c.6542334 |
| Popis: |
Pancreatic ductal adenocarcinoma (PDAC) is an incurable, highly metastatic disease that is largely resistant to existing treatments. A better understanding of the genetic basis of PDAC metastasis should facilitate development of improved therapies. To that end, we developed a novel mouse xenograft model of PDAC metastasis to expedite testing of candidate genes associated with the disease. Human PDAC cell lines BxPC-3, MiaPaCa-2, and Panc-1 stably expressing luciferase were generated and introduced by intracardiac injections into immunodeficient mice to model hematogenous dissemination of cancer cells. Tumor development was monitored by bioluminescence imaging. Bioluminescent MiaPaCa-2 cells most effectively recapitulated PDAC tumor development and metastatic distribution in vivo. Tumors formed in nearly 90% of mice and in multiple tissues, including normal sites of PDAC metastasis. Effects of p14ARF, a known suppressor of PDAC, were tested to validate the model. In vitro, p14ARF acted through a CtBP2-dependent, p53-independent pathway to inhibit MiaPaCa-2–invasive phenotypes, which correlated with reduced tumor cell colonization in vivo. These findings establish a new bioluminescent mouse tumor model for rapidly assessing the biological significance of suspected PDAC metastasis genes. This system may also provide a valuable platform for testing innovative therapies. Mol Cancer Res; 9(7); 867–77. ©2011 AACR. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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