Identification of Polygonum orientale constituents using high-performance liquid chromatography high-resolution tandem mass spectrometry
| Autor: | Young Ho Jeon, Xi-Tao Yan, Yeeun Park, Hyeji Shin, Ki Yong Lee |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Antioxidant
medicine.drug_class medicine.medical_treatment Anti-Inflammatory Agents Tandem mass spectrometry Mass spectrometry 01 natural sciences Applied Microbiology and Biotechnology Biochemistry High-performance liquid chromatography Antioxidants Anti-inflammatory Analytical Chemistry Column chromatography Tandem Mass Spectrometry Cell Line Tumor medicine Animals Viability assay Molecular Biology Chromatography High Pressure Liquid Chromatography 010405 organic chemistry Chemistry Organic Chemistry Biological activity General Medicine 0104 chemical sciences 010404 medicinal & biomolecular chemistry Neuroprotective Agents Polygonum Drugs Chinese Herbal Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 82:15-21 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1080/09168451.2017.1415124 |
| Popis: | Our primary focus in this research was to identify and characterize its bioactive compounds for potential therapeutic use. Twenty-seven metabolites of Polygonum orientale were identified using LC-QTOF tandem mass spectrometry. Interestingly, P. orientale extracts included several highly oxygenated flavonoids were isolated from P. orientale by column chromatography. 13C NMR data of highly oxygenated flavonoids (1–7) are reported here for the first time. In addition, nitric oxide, 1,1-diphenyl-2-picrylhydrazyl, and water-soluble tetrazolium salt assays were carried out on the isolated compounds to investigate their anti-inflammatory, anti-oxidant, and neuroprotective activities, respectively. Compounds 1, 2, 3, 5, 7, and 8 significantly attenuated lipopolysaccharide-stimulated NO production in BV2 cells without affecting cell viability. Compounds 9–12 exhibited significant antioxidant activity, while compounds 8, 9, and 12 exhibited protective effects against glutamate-induced neurotoxicity in HT22 cells. Our results indicate that P. orientale is a promising source of natural agents for the potential treatment of inflammation and neurodegenerative diseases. Chemical profile of Polygonum orientale. |
| Databáze: | OpenAIRE |
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