Demonstration of the requirements for cholesterol or low density lipoprotein in the antibody response of murine lymphocytes in serum-free culture
| Autor: | Shigehiro Takahama, Hitoshi Ohmori |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
chemistry.chemical_classification
biology Cholesterol Albumin High-performance liquid chromatography Primary and secondary antibodies In vitro General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound chemistry Biochemistry Transferrin Cell culture Low-density lipoprotein biology.protein lipids (amino acids peptides and proteins) General Agricultural and Biological Sciences |
| Zdroj: | Agricultural and Biological Chemistry. 53:209-213 |
| ISSN: | 1881-1280 0002-1369 |
| DOI: | 10.1271/bbb1961.53.209 |
| Popis: | We report the essential role of lipid in the development of a serum-free culture medium that supports the primary antibody response by murine lymphocytes in vitro. In our preceding paper, we have reported that β-cyclodextrin (β-CD) is effective as a serum-substitute in murine lymphocyte cultures [H. Ohmori and I. Yamamoto, Eur. J. Immunol., 17, 79 (1987)]. At first, we employed RPMI-1640 that was supplemented with fatty acid-free albumin, insulin, transferrin, and β-CD as the basal serum-free medium (BSF medium). BSF medium supported the antibody response less than 30 % as efficiently as 10% fetal calf serum (FCS)-containing medium. When ether-extracts of FCS (FCS ex.) was added to BSF medium, the antibody response was enhanced by approximately 2-fold. FCS ex. was purified by silica gel thin-layer chromatography. The analyses of the purified active component by high performance liquid chromatography and mass spectrometry revealed it to be cholesterol. Authentic cholesterol or low density lipoprotein enhan... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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