Isolation and Characterization of Bipotent Liver Progenitor Cells from Adult Mouse
| Autor: | Joseph T.Y. Lau, Yu-Cheng Yao, Juan Su, Yi-Ping Hu, Xin-Min Wang, Yong-Bi Yan, Hong-Yu Yu, Xin-Rong Tao, Jian-Xiu Li, Yong-Ji Yang, Wenlin Li |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Cell Transplantation Cellular differentiation CD34 Liver Stem Cell Biology Transfection Mice medicine Animals Hepatectomy Humans Progenitor cell Pyrrolizidine Alkaloids Cell Proliferation Matrigel Stem Cells Cell Differentiation Epithelial Cells Cell Biology Liver regeneration Liver Regeneration Cell biology Mice Inbred C57BL Liver Immunology Hepatocytes NIH 3T3 Cells Molecular Medicine Hepatocyte growth factor Bile Ducts Stem cell Biomarkers HeLa Cells Transcription Factors Developmental Biology medicine.drug |
| Zdroj: | Stem Cells. 24:322-332 |
| ISSN: | 1549-4918 1066-5099 |
| DOI: | 10.1634/stemcells.2005-0108 |
| Popis: | Liver progenitor cells have drawn a great deal of attention both for their therapeutic potential and for their usefulness in exploring the molecular events surrounding liver development and regeneration. Despite the intensive studies on liver progenitors from rats, equivalent progenitor cells derived from mice are relatively rare. We used retrosine treatment followed by partial hepatectomy to elicit liver progenitors in mice. From these animals showing prominent ductular reactions, mouse-derived liver progenitor cell lines (LEPCs) were isolated by single-cell cloning. Phenotypic and lineage profiling of the LEPC clones were performed using immunochemistry, reverse transcription–polymerase chain reaction, and a dual-color system comprising the reporter EGFP under the control of the cytokeratin 19 promoter and the DsRed reporter under the control of the albumin promoter. LEPCs expressed liver progenitor cell markers. LEPCs also expressed some markers shared by bone marrow-derived hematopoietic stem cells c-Kit and Thy-1 but not CD34 and CD45. When cultured as aggregates in Matrigel, LEPCs differentiated into hepatocyte upon treatment with 50 ng/ml epithelial growth factor or differentiated into biliary lineage cells upon treatment with 20 ng/ml hepatocyte growth factor. In the presence of 2% dimethyl sulfoxide and 2% Matrigel, LEPCs acquired predominantly bile lineage phenotypes, with occasional patches of cells exhibiting hepatocyte phenotypes. Upon transplantation into CCl4-injured-liver, LEPCs engrafted into liver parenchyma and differentiated into hepatocytes. Considering the amenability of the mouse to genetic manipulation, these mouse-derived LEPCs may be useful tools as in vitro models to study molecular events in liver development and regeneration and can shed light in studying the therapy potential of liver stem cells. |
| Databáze: | OpenAIRE |
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