Studies by DNA-RNA hybridization of transcriptional diversity in human brain
| Autor: | L. Grouse, B. J. McCarthy, G. A. Omenn |
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| Předmět: |
Male
Pathology medicine.medical_specialty Transcription Genetic DNA Single-Stranded Growth Biology Astrocytoma Tritium Biochemistry Genome Cellular and Molecular Neuroscience chemistry.chemical_compound Neuroblastoma Fetus Pregnancy Culture Techniques medicine Humans Cells Cultured Aged Temporal cortex Cerebral atrophy RNA Brain Nucleic Acid Hybridization Human brain Middle Aged medicine.disease Molecular biology Kinetics medicine.anatomical_structure chemistry Nucleic Acid Renaturation Female Autopsy DNA HeLa Cells |
| Zdroj: | Scopus-Elsevier |
| Popis: | — Purified unique sequences of human DNA (from HeLa cells) were hybridized with various preparations of human brain KNA, to obtain estimates of the fraction of DNA transcribed. Values of 12 per cent were obtained for fetal brain increasing to 24 per cent for specific regions of adult brain. Right and left parietal and temporal cortical RNA gave similar plateau values but left frontal cortical RNA appeared to represent 2–3 times as much of the total genome as that from right frontal cortex. Lower values were obtained for fetal and adult brain stem and for RNA prepared from temporal cortex of a patient exhibiting gross cerebral atrophy. Attempts were made to assess the contributions of glial and neuronal cells by using human astrocytoma and mouse neuroblastoma RNA. |
| Databáze: | OpenAIRE |
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