Modulation of the immune response to ragweed allergens by peptic fragments
| Autor: | J. G. Michael, Allen Litwin, Amadeo J. Pesce, V. Hassert |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Ragweed
Allergen immunotherapy Allergy T-Lymphocytes Immunology medicine.disease_cause Lymphocyte Activation Microbiology Mice Immune system Allergen Antigen medicine Immunology and Allergy Animals Immunosuppression Therapy biology Immunogenicity Immunoglobulin E biology.organism_classification medicine.disease Peptide Fragments Molecular Weight Desensitization Immunologic biology.protein Pollen Female Immunization Antibody |
| Zdroj: | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology. 20(6) |
| ISSN: | 0954-7894 |
| Popis: | Summary Fragments of short ragweed extract were prepared by peptic digestion and fractionated by molecular exclusion chromatography. We focused on fragmenting a ragweed fraction that was enriched for Amb a I. Digestion products in the molecular weight range of 5–15 kD (fSRW) were examined for their antigenic, immunogenic and immunosuppressive properties. fSRW was poorly immunogenic and unable to induce ragweed-specific PCA reactions in rat skin or to bind to anti-ragweed IgG antibodies. Intravenous administration of fSRW either prior to or after intraperitoneal immunization with a ragweed preparation resulted in a significant suppression of the immune response. T cells isolated from lymph nodes of mice immunized with ragweed were stimulated by fSRW in a lymphoproliferation assay. Moreover, T cells from mice injected with fSRW were immunosuppressive when transferred into mice immunized with intact ragweed antigens. Our data demonstrate that peptic fragments (fSRW) of ragweed lack B-cell determinants while retaining their T-cell immunoregulatory properties. Potentially these peptides are less likely to cause adverse reactions in allergen sensitive patients and thus offer a new approach to allergen immunotherapy. |
| Databáze: | OpenAIRE |
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