Antimicrobial response of galectin-1 from rock bream Oplegnathus fasciatus: Molecular, transcriptional, and biological characterization
| Autor: | Navaneethaiyer Umasuthan, Ilson Whang, Jehee Lee, William Shanthakumar Thulasitha, Bo-Hye Nam |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Fish Proteins
Lipopolysaccharides 0301 basic medicine Vibrio anguillarum Galectin 1 Aquatic Science Conserved sequence Microbiology Fish Diseases 03 medical and health sciences 0302 clinical medicine Streptococcal Infections Animals Environmental Chemistry Streptococcus iniae Amino Acid Sequence Edwardsiella tarda Phylogeny Galectin Innate immune system biology Vibrio harveyi Enterobacteriaceae Infections Pattern recognition receptor General Medicine biology.organism_classification DNA Virus Infections Immunity Innate Iridoviridae Perciformes Protein Structure Tertiary Poly I-C 030104 developmental biology Organ Specificity Sequence Alignment 030215 immunology |
| Zdroj: | Fish & Shellfish Immunology. 50:66-78 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2016.01.010 |
| Popis: | In this study, we describe the identification and characterization of a proto type galectin, galectin-1, from rock bream Oplegnathus fasciatus (OfGal-1). Galectins are evolutionarily conserved carbohydrate binding lectins that show a wide range of functions related to development and immune physiology. They have been identified as pattern recognition receptors of innate immune system that recognize a broad range of microbes. OfGal-1 cDNA comprised of 993 bp with an open reading frame of 408 bp that encodes 135 amino acids. A single carbohydrate recognition domain was present in the OfGal-1 amino acid sequence. The sequence comparison by multiple and pairwise alignments and the phylogenetic tree emphasized the strong evolutionary conservation of Gal-1. The typical β-sandwich structure was identified from the predicted tertiary structure. The constitutive expression of mRNA transcripts was detected in a wide range of tissues examined, with the highest expression in the heart. Immune challenges with live bacteria (Edwardsiella tarda and Streptococcus iniae), rock bream irido virus, and mitogens (lipopolysaccharide and poly I:C) modulated the expression of OfGal-1 mRNAs in the gills, head kidney, and liver. The recombinant OfGal-1 (rOfGal-1) strongly agglutinatinated the human erythrocytes, and this hemagglutination was inhibited by lactose and D-galactose. A wide range of bacteria (S. iniae, S. parauberis, Escherichia coli, Edwardsiella tarda, Vibrio anguillarum, Vibrio harveyi, and Vibrio tapetis) and a ciliate (Miamiensis avidus) were also effectively recognized by rOfGal-1. Significant antiviral activity against rock bream irido virus was also demonstrated by rOfGal-1. Collectively, results from the present study indicate that OfGal-1 can recognize a wide range of microbes and is a vital pattern recognition receptor in the innate immune system of rock bream. |
| Databáze: | OpenAIRE |
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