Maternal Dietary Protein Intake Influences Milk and Offspring Gut Microbial Diversity in a Rat (Rattus norvegicus) Model
| Autor: | Keah V. Higgins, Wendy R. Hood, Haley Hallowell, Matthew F Warren, Mark R. Liles |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Offspring 030106 microbiology Population lcsh:TX341-641 phylogenetic distance 03 medical and health sciences fluids and secretions Lactobacillus Lactation Milk microorganisms medicine Food science Microbiome education Feces 16S rRNA gene sequencing 2. Zero hunger education.field_of_study Nutrition and Dietetics biology Maternal effect food and beverages bacteria diversity biology.organism_classification 030104 developmental biology medicine.anatomical_structure Microbial population biology lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Nutrients, Vol 11, Iss 9, p 2257 (2019) Nutrients Volume 11 Issue 9 |
| ISSN: | 2072-6643 |
| Popis: | Historically, investigators have assumed microorganisms identified in mother&rsquo s milk to be contaminants, but recent data suggest that milk microbiota may contribute to beneficial maternal effects. Microorganisms that colonize the gastrointestinal tracts of newborn mammals are derived, at least in part, from the maternal microbial population. Milk-derived microbiota is an important source of this microbial inocula and we hypothesized that the maternal diet contributes to variation in this microbial community. To evaluate the relationship between a mother&rsquo s diet and milk microbiome, we fed female rats a low- or high-protein diet and mated all individuals. Milk and cecal contents were collected from dams at peak lactation (14-day post-partum), and the bacterial composition of each community was assessed by 16S rRNA gene amplicon sequencing. Our findings revealed higher dietary protein intake decreased fecal microbial diversity but increased milk microbial and pup cecum diversity. Further, the higher dietary protein intake resulted in a greater abundance of potentially health-promoting bacteria, such as Lactobacillus spp. These data suggest that dietary protein levels contribute to significant shifts in the composition of maternal milk microbiota and that the functional consequences of these changes in microbial inocula might be biologically important and should be further explored. |
| Databáze: | OpenAIRE |
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