Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories
| Autor: | Biljana Jančić-Stojanović, Andjelija Malenovic, Darko Ivanović, Mirjana Medenica, Slavko Marković |
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| Rok vydání: | 2010 |
| Předmět: |
Hydrocortisone
Lidocaine Anti-Inflammatory Agents Hemorrhoids 030226 pharmacology & pharmacy 01 natural sciences High-performance liquid chromatography Analytical Chemistry 03 medical and health sciences 0302 clinical medicine medicine Humans Environmental Chemistry Response surface methodology Anesthetics Local Chromatography High Pressure Liquid Hydrocortisone acetate Pharmacology Analysis of Variance Chromatography Reverse-Phase Chromatography Chemistry Methanol Suppositories 010401 analytical chemistry Hydrogen-Ion Concentration 0104 chemical sciences Uv detection Selectivity Agronomy and Crop Science Food Science medicine.drug |
| Zdroj: | Journal of AOAC International Scopus-Elsevier |
| Popis: | An RP-HPLC method has been optimized and validated for the simultaneous determination of hydrocortisone acetate and of lidocaine in suppositories. For the method optimization, response surface methodology was applied, and the obtained model was tested using analysis of variance. The optimal separations were conducted on a Beckman-Coulter 150 4.6 mm, 5 µm particle-size column at 20C. The mobile phase was methanolwater (65 + 35, v/v), pH adjusted to 2.5 with 85% orthophosphoric acid, with a flow rate of 1.0 mL/min. UV detection was performed at 250 nm. Phenobarbital was used as an internal standard. The method was validated for selectivity, linearity, precision, and robustness. |
| Databáze: | OpenAIRE |
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