A DNA-Binding Copper(I) Metallosupramolecular Cylinder that Acts as an Artificial Nuclease
| Autor: | Michael J. Hannon, Mark J. Broome, Laura J. Childs, Virtudes Moreno, Mirela Pascu, Britt Elin Rolfsnes, Alison Rodger, P. Mark Rodger, Einar Sletten, Jaroslav Malina, María J. Prieto |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Nuclease
Deoxyribonucleases biology Pyridines Chemistry Stereochemistry Organic Chemistry Supramolecular chemistry DNA General Chemistry Ligand (biochemistry) Linear dichroism Catalysis Dication Crystallography chemistry.chemical_compound Cleave Organometallic Compounds biology.protein A-DNA DNA Cleavage Copper |
| Zdroj: | Chemistry - A European Journal. 12:4919-4927 |
| ISSN: | 1521-3765 0947-6539 |
| DOI: | 10.1002/chem.200600060 |
| Popis: | The DNA binding of a dicationic pyridylimine-based dicopper(i) metallosupramolecular cylinder is reported together with its ability to act as an artificial nuclease. The cylinder binds strongly to DNA; more strongly than the spherical dication [Ru(phen)(3)](2+) (phen = 1,10-phenanthroline), but more weakly than the corresponding tetracationic cylinders. DNA coiling effects are not observed with this dication, in contrast to the situation with the previously reported tetracationic cylinder involving a similar ligand. Linear dichroism (LD) data suggests that the dicopper cylinder binds in a different orientation from that of the tetracationic iron cylinder. Furthermore, the dicopper cylinder shows DNA-cleavage activity in the presence of peroxide. Of particular note is that the cylinder displays a marked and unusual ability to cleave both DNA strands at the same site, probably reflecting its dinuclear nature and possibly its mode of binding to the DNA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text