Stress-Dependent Nucleolin Mobilization Mediated by p53-Nucleolin Complex Formation
| Autor: | James A. Borowiec, Diana D. Dimitrova, Yaron Daniely |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
DNA replication initiation
DNA repair Nucleolus Active Transport Cell Nucleus Biology Cell Fractionation Transactivation Radiation Ionizing Tumor Cells Cultured Humans Enzyme Inhibitors Nuclear protein Cell Growth and Development Lung Molecular Biology Replication protein A Cell Nucleus Nucleoplasm Nuclear Proteins RNA-Binding Proteins Cell Biology Fibroblasts Phosphoproteins Cell biology Microscopy Fluorescence Camptothecin Tumor Suppressor Protein p53 Nucleolin |
| Zdroj: | Molecular and Cellular Biology. 22:6014-6022 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.22.16.6014-6022.2002 |
| Popis: | We recently discovered that heat shock causes nucleolin to relocalize from the nucleolus to the nucleoplasm, whereupon it binds replication protein A and inhibits DNA replication initiation. We report that nucleolin mobilization also occurs following exposure to ionizing radiation (IR) and treatment with camptothecin. Mobilization was selective in that another nucleolar marker, upstream binding factor, did not relocalize in response to IR. Nucleolin relocalization was dependent on p53 and stress, the latter initially stimulating nucleolin-p53 complex formation. Nucleolin relocalization and complex formation in vivo were independent of p53 transactivation but required the p53 C-terminal regulatory domain. Nucleolin and p53 also interact directly in vitro, with a similar requirement for p53 domains. These data indicate a novel p53-dependent mechanism in which cell stress mobilizes nucleolin for transient replication inhibition and DNA repair. |
| Databáze: | OpenAIRE |
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