Scanning Electron Microscope: A New Potential Tool to Replace Gram Staining for Microbe Identification in Blood Cultures
| Autor: | Gabriel Haddad, Yusuke Ominami, Anthony Fontanini, T. Takakura, Sara Bellali, Didier Raoult, Jacques Yaacoub Bou Khalil |
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| Přispěvatelé: | Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Hitachi, Ltd, Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), ANR-10-IAHU-0003,Méditerranée Infection,I.H.U. Méditerranée Infection(2010), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) MALDI-TOF QH301-705.5 Scanning electron microscope 030106 microbiology microbe identification blood culture Microbiology Article law.invention 03 medical and health sciences rapid test [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system law [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Virology medicine Blood culture Sample preparation [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology Biology (General) Gram [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases gram medicine.diagnostic_test biology Gold standard (test) biology.organism_classification [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology 3. Good health 030104 developmental biology Gram staining [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Identification (biology) Bacteria scanning electron microscopy |
| Zdroj: | Microorganisms Microorganisms, 2021, 9 (6), pp.1170. ⟨10.3390/microorganisms9061170⟩ Microorganisms, MDPI, 2021, 9 (6), pp.1170. ⟨10.3390/microorganisms9061170⟩ Volume 9 Issue 6 Microorganisms, Vol 9, Iss 1170, p 1170 (2021) |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms9061170⟩ |
| Popis: | International audience; Blood culture is currently the most commonly used method for diagnosing sepsis and bloodstream infections. However, the long turn-around-time to achieve microbe identification remains a major concern for clinical microbiology laboratories. Gram staining for preliminary identification remains the gold standard. We developed a new rapid strategy using a tabletop scanning electron microscope (SEM) and compared its performance with Gram staining for the detection of micro-organisms and preliminary identification directly from blood cultures. We first optimised the sample preparation for twelve samples simultaneously, saving time on imaging. In this work, SEM proved its ability to identify bacteria and yeasts in morphotypes up to the genus level in some cases. We blindly tested 1075 blood cultures and compared our results to the Gram staining preliminary identification, with MALDI-TOF/MS as a reference. This method presents major advantages such as a fast microbe identification, within an hour of the blood culture being detected positive, low preparation costs, and data traceability. This SEM identification strategy can be developed into an automated assay from the sample preparation, micrograph acquisition, and identification process. This strategy could revolutionise urgent microbiological diagnosis of infectious diseases. |
| Databáze: | OpenAIRE |
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