Ontogeny and regulation of the AT1 and AT2 receptors in the ovine fetal adrenal gland
| Autor: | Karen M. Moritz, E. M. Wintour, N. Tenis, Anthony L. Albiston, A. Butkus, R Baird |
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| Rok vydání: | 1999 |
| Předmět: |
endocrine system
medicine.medical_specialty Blotting Western Gestational Age Stimulation Biology Receptor Angiotensin Type 2 Biochemistry Receptor Angiotensin Type 1 chemistry.chemical_compound Fetus Ribonucleases Endocrinology Zona fasciculata Internal medicine Adrenal Glands medicine Animals RNA Messenger Steroid 11-beta-hydroxylase Receptor Molecular Biology In Situ Hybridization Receptors Angiotensin Sheep Aldosterone Angiotensin II receptor type 1 Angiotensin II Gene Expression Regulation Developmental Glyceraldehyde-3-Phosphate Dehydrogenases Immunohistochemistry medicine.anatomical_structure chemistry Zona glomerulosa hormones hormone substitutes and hormone antagonists |
| Zdroj: | Molecular and Cellular Endocrinology. 157:161-170 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/s0303-7207(99)00149-5 |
| Popis: | The expression and regulation of the receptors for angiotensin II (both AT1 and AT2) were examined in the ovine fetal adrenal gland by RNase protection assay (RPA), in situ hybridisation histochemistry, immunohistochemistry and Western blotting. Both mRNA and protein for the AT1 receptor were present in the zona glomerulosa and zona fasciculata of the cortex, but not in the medulla, from as early as these zonas were distinguishable (60 days of gestation; term is 145–150 days), and even present in the steroidogenic cells of the unzoned gland at 40 days. The mRNA for the AT2 receptor was present in the same locations (but never in the medulla) from 40–130 days, and declined to extremely low levels after 140 days. The infusion of ang II, 1 μg/h, for 3 days, at mid-gestation (76±2 days) caused a significant decrease in mRNA for AT1 but no change in AT2 levels. Thus, the biologically active receptor (in terms of aldosterone stimulation) is present in the ovine fetal adrenal from very early in development, and can be down-regulated by mid-gestation. |
| Databáze: | OpenAIRE |
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