Resistance to systemic inflammation and multi organ damage after global ischemia/reperfusion in the arctic ground squirrel
| Autor: | Jasmine M. Olson, JoAnna Carpluk, Kelly L. Drew, Jeanette M. Moore, Lori K. Bogren |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Critical Care and Emergency Medicine Physiology Cardiovascular Procedures lcsh:Medicine Cardiovascular Physiology Pathology and Laboratory Medicine Systemic inflammation Biochemistry Vascular Medicine Medicine and Health Sciences lcsh:Science Trauma Medicine Multidisciplinary Sciuridae Hematology Animal Models Stroke Hemorrhagic Stroke Neurology Research Design Reperfusion Injury Blood Circulation Multiple Organ Dysfunction Syndrome Female Anatomy medicine.symptom Perfusion Research Article medicine.medical_specialty Mean arterial pressure Clinical Research Design Resuscitation Cerebrovascular Diseases Multiple Organ Failure Trauma Surgery Cardiology Ischemia Surgical and Invasive Medical Procedures Inflammation Biology Research and Analysis Methods Signs and Symptoms Model Organisms Internal medicine medicine Animals Gastrointestinal Critical Care Animal Models of Disease Ischemic Stroke Acute Cardiovascular Problems lcsh:R Hemodynamics Biology and Life Sciences medicine.disease Rats Health Care Metabolism Blood pressure Endocrinology Immunology Cardiovascular Anatomy Base excess lcsh:Q Energy Metabolism Physiological Processes Reperfusion injury |
| Zdroj: | PLoS ONE, Vol 9, Iss 4, p e94225 (2014) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Introduction Cardiac arrest (CA) and hemorrhagic shock (HS) are two clinically relevant situations where the body undergoes global ischemia as blood pressure drops below the threshold necessary for adequate organ perfusion. Resistance to ischemia/reperfusion (I/R) injury is a characteristic of hibernating mammals. The present study sought to determine if arctic ground squirrels (AGS) are protected from systemic inflammation and multi organ damage after CA- or HS-induced global I/R and if, for HS, this protection is dependent upon their hibernation season. Methods For CA, rats and summer euthermic AGS (AGS-EU) were asphyxiated for 8 min, inducing CA. For HS, rats, AGS-EU, and winter interbout arousal AGS (AGS-IBA) were subject to HS by withdrawing blood to a mean arterial pressure of 35 mmHg and maintaining that pressure for 20 min before reperfusion with Ringers. For both I/R models, body temperature (Tb) was kept at 36.5–37.5°C. After reperfusion, animals were monitored for seven days (CA) or 3 hrs (HS) then tissues and blood were collected for histopathology, clinical chemistries, and cytokine level analysis (HS only). For the HS studies, additional groups of rats and AGS were monitored for three days after HS to access survival and physiological impairment. Results Rats had increased serum markers of liver damage one hour after CA while AGS did not. For HS, AGS survived 72 hours after I/R whereas rats did not survive overnight. Additionally, only rats displayed an inflammatory response after HS. AGS maintained a positive base excess, whereas the base excess in rats was negative during and after hemorrhage. Conclusions Regardless of season, AGS are resistant to organ damage, systemic inflammation, and multi organ damage after systemic I/R and this resistance is not dependent on their ability to become decrease Tb during insult but may stem from an altered acid/base and metabolic response during I/R. |
| Databáze: | OpenAIRE |
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