An apple B-box protein, MdCOL11, is involved in UV-B- and temperature-induced anthocyanin biosynthesis
| Autor: | Takaya Moriguchi, Takanori Saito, Songling Bai, Chikako Honda, Yoshimichi Hatsuyama, Akiko Ito |
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| Rok vydání: | 2014 |
| Předmět: |
Ultraviolet Rays
Molecular Sequence Data Arabidopsis Pancreatitis-Associated Proteins Flowers Plant Science Hypocotyl Anthocyanins chemistry.chemical_compound Gene Expression Regulation Plant Genetics MYB Amino Acid Sequence Phylogeny Plant Proteins Regulation of gene expression Models Genetic Plant Stems Sequence Homology Amino Acid biology Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling fungi Temperature food and beverages Ripening Plants Genetically Modified biology.organism_classification Biochemistry chemistry Fruit Malus Anthocyanin bacteria Photomorphogenesis Petal |
| Zdroj: | Planta. 240:1051-1062 |
| ISSN: | 1432-2048 0032-0935 |
| DOI: | 10.1007/s00425-014-2129-8 |
| Popis: | Our studies showed that an apple B-box protein, MdCOL11, the homolog of AtBBX22, is involved in UV-B- and temperature-induced anthocyanin biosynthesis in apple peel. Anthocyanin is responsible for the red pigmentation in apple peel and a R2R3 MYB gene, MdMYBA/1/10, a homolog of MdMYBA, controls its accumulation. Arabidopsis PAP1 is under the control of a series of upstream factors involved in light signal transduction and photomorphogenesis, such as ELONGATED HYPOCOTYL 5 (HY5) and B-box family (BBX) proteins. In this study, we identified and characterized the homolog of Arabidopsis BBX22 in apple, designated as MdCOL11. Overexpression of MdCOL11 in Arabidopsis enhanced the accumulation of anthocyanin. In apples, MdCOL11 was differentially expressed in all tissues, with the highest expression in petals and the lowest expression in the xylem. Transcripts of MdCOL11 noticeably accumulated at the ripening stage, concomitant with increases in the expressions of anthocyanin biosynthesis-related genes. In an in vitro treatment experiment, MdCOL11 was upregulated in an ultra-violet (UV)-B- and temperature-dependent manner, together with the inductions of anthocyanin biosynthesis-related genes and anthocyanin accumulation in apple peel. Furthermore, a dual-luciferase assay indicated that (1) MdCOL11 regulated the expression of MdMYBA and (2) MdCOL11 was a target of MdHY5. Taken together, our results suggest that MdCOL11 is involved in MdHY5-mediated signal transduction and regulates anthocyanin accumulation in apple peel, which sheds new light on anthocyanin accumulation in apples. |
| Databáze: | OpenAIRE |
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