Molecular characterization and structural instability of the industrially important composite metabolic plasmid pLP712

Autor: Karin Overweg, M. J. Gasson, Sophie Jeanson, Udo Wegmann, Claire Shearman
Přispěvatelé: Institute of Food Research, Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Biotechnology and Biological Sciences Research Council (BBSRC) Core Strategic Grant. S. J. was supported by EU Marie Curie Fellowship OXYRED O41161, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Rok vydání: 2012
Předmět:
Lactococcus
Sequence Homology
lac operon
casein
Plasmid
[SDV.IDA]Life Sciences [q-bio]/Food engineering
lactococcus
Replicon
industrie laitière
Insertion sequence
fermentation
Recombination
Genetic
Genetics
milk
biology
food and beverages
milk industry
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Biochemistry
Conjugation
Genetic
plasmide
Metabolic Networks and Pathways
Plasmids
DNA
Bacterial
bacterial genes
Gene Transfer
Horizontal
Molecular Sequence Data
milk sugar
gène bactérien
fermented foods
Microbiology
Genomic Instability
Evolution
Molecular
lactose
serine
Industrial Microbiology
Transformation
Genetic
catabolisme
lactococcus lactis
plasmid
analyse moléculaire
Gene
caséine
gène
aliment fermenté
Lactococcus lactis
Sequence Analysis
DNA
biology.organism_classification
lait
protéinase
Homologous recombination
metabolism
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Zdroj: Microbiology / Mikrobiologiya
Microbiology / Mikrobiologiya, MAIK Nauka/Interperiodica, 2012, 158, pp.2936-2945. ⟨10.1099/mic.0.062554-0⟩
ISSN: 1465-2080
1350-0872
0026-2617
1608-3237
Popis: The widely used plasmid-free Lactococcus lactis strain MG1363 was derived from the industrial dairy starter strain NCDO712. This strain carries a 55.39 kb plasmid encoding genes for lactose catabolism and a serine proteinase involved in casein degradation. We report the DNA sequencing and annotation of pLP712, which revealed additional metabolic genes, including peptidase F, D-lactate dehydrogenase and a-keto acid dehydrogenase (E3 complex). Comparison of pLP712 with other large lactococcal lactose and/or proteinase plasmids from L. lactis subsp. cremoris SK11 (pSK11L, pSK11P) and the plant strain L. lactis NCDO1867 (pGdh442) revealed their close relationship. The plasmid appears to have evolved through a series of genetic events as a composite of pGdh442, pSK11L and pSK11P. We describe in detail a scenario by which the metabolic genes relevant to the growth of its host in a milk environment have been unified on one replicon, reflecting the evolution of L. lactis as it changed its biological niche from plants to dairy environments. The extensive structural instability of pLP712 allows easy isolation of derivative plasmids lacking genes for casein degradation and/or lactose catabolism. Plasmid pLP712 is transferable by transduction and conjugation, and both of these processes result in significant molecular rearrangements. We report the detailed molecular analysis of insertion sequence element-mediated genetic rearrangements within pLP712 and several different mechanisms, including homologous recombination and adjacent deletion. Analysis of the integration of the lactose operon into the chromosome highlights the fluidity of the MG1363 integration hotspot and the potential for frequent movement of genes between plasmids and chromosomes in Lactococcus.
Databáze: OpenAIRE
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