Relative transcriptional activities ofSAA1promoters polymorphic at position −13(T/C): Potential association between increased transcription and amyloidosis
| Autor: | Shinichi Inada, Chihiro Terai, Yutaka Kitamura, Yumi Koseki, Hiroshi Kajiyama, Naoyuki Kamatani, Masato Moriguchi, Hirotaka Kaneko |
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| Rok vydání: | 2005 |
| Předmět: |
Carcinoma
Hepatocellular Transcription Genetic Population Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide White People Arthritis Rheumatoid Asian People Tumor Cells Cultured Internal Medicine Humans SNP Serum amyloid A Allele Luciferases Promoter Regions Genetic education Gene Alleles Genetics Serum Amyloid A Protein education.field_of_study Liver Neoplasms Haplotype Promoter Amyloidosis Molecular biology Haplotypes Plasmids |
| Zdroj: | Amyloid. 12:26-32 |
| ISSN: | 1744-2818 1350-6129 |
| DOI: | 10.1080/13506120500032394 |
| Popis: | The risk associated with the serum amyloid A (SAA) 1 gene and developing AA-amyloidosis is still controversial. In familial Mediterranean fever or Caucasoid rheumatoid arthritis (RA), the SAA1.1 allele is a risk factor for the development of AA-amyloidosis. However, individuals with the SAA1.3 allele are susceptible to AA-amyloidosis in the Japanese RA population, but those with the SAA1.1 are not. Previous reports have indicated that the -13T/C single nucleotide polymorphism (SNP) at the 5'-flanking region of SAA1 appears to be a better marker of AA-amyloidosis than the exon-3 based haplotype, i.e., SAA1.1 or SAA1.3, in both Japanese and American Caucasian populations. So far, it is unknown why the -13T SNP increases the amyloidogenicity of the patients. In the present study, a luciferase reporter gene assay showed that the transcriptional activity of the SAA1 having the -13T-containing promoter was significantly higher than activities of those with -13C-containing promoters (Fisher's protected least significance difference test). We suggest that having the -13T SNP in the SAA1 promoter correlates with the amyloidogenicity in part as a result of this increased transcriptional activity. |
| Databáze: | OpenAIRE |
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