Protective effects of Weipixiao decoction against MNNG-induced gastric precancerous lesions in rats
Autor: | Xiaohui Zeng, Huafeng Pan, Chengzhe Zhang, Lei Wu, Xuhua Yu, Lin Lin, Ziming Zhao, Tiantian Cai, Yan Yan |
---|---|
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Male Methylnitronitrosoguanidine Decoction RM1-950 Precancerous gastric lesions Gene Expression Regulation Enzymologic Pathogenesis Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Western blot Stomach Neoplasms Medicine Animals Anticarcinogenic Agents Glycolysis Protein kinase B PI3K/AKT/mTOR pathway Pharmacology medicine.diagnostic_test business.industry Stomach Cancer General Medicine medicine.disease Rats Gene Expression Regulation Neoplastic Disease Models Animal 030104 developmental biology Weipixiao Cytoprotection 030220 oncology & carcinogenesis Cancer research Immunohistochemistry Therapeutics. Pharmacology business Precancerous Conditions Drugs Chinese Herbal Signal Transduction |
Zdroj: | Biomedicine & Pharmacotherapy, Vol 120, Iss, Pp-(2019) |
ISSN: | 1950-6007 |
Popis: | Gastric cancer is recognized as one of the most common cancer. In-depth research of gastric precancerous lesions (GPL) plays an important role in preventing the occurrence of gastric cancer. Meanwhile, traditional treatment provides a novel sight in the prevention of occurrence and development of gastric cancer. The current study was designed to assess the effects of therapy with Weipixiao (WPX) decoction on N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced GPL rats and the underlying molecular mechanisms. After 10-weeks treatment, all rats were sacrificed. Histopathological changes of gastric tissue were assessed via hematoxylin-eosin (HE) and High-iron diamine-Alcian blue-Periodic acid-Schiff (HID-AB-PAS) staining. To be fully evidenced, RT-qPCR, Western blot and immunohistochemistry were used to detect the expressions of LDHA, CD147, HIF-1α, MCT4, PI3K, AKT, mTOR and miRNA-34a, which were crucial factors for evaluating GPL in the aspect of glycolysis pathogenesis. According to the results of HE and HID-AB-PAS staining, it could be confirmed that MNNG-induced GPL rats were obviously reversed by WPX decoction. Additionally, the increased gene levels of LDHA, CD147, MCT4, PI3K, AKT, mTOR and HIF-1α in model group were down-regulated by WPX decoction, while miRNA-34a expression was decreased and up-regulated by WPX decoction. The significantly increased protein levels of LDHA, CD147, MCT4, PI3K, AKT, mTOR and HIF-1α induced by MNNG were attenuated in rats treated with WPX decoction. In brief, the findings of this study imply that abnormal glycolysis in MNNG-induced GPL rats was relieved by WPX decoction via regulation of the expressions of LDHA, CD147, HIF-1α, MCT4, PI3K, AKT, mTOR and miRNA-34a. |
Databáze: | OpenAIRE |
Externí odkaz: |