Evaluation of Nested Polymerase Chain Reaction for Rapid Diagnosis of Clinically Suspected Tuberculous Pleurisy
| Autor: | Sahiba Kukreja, Namrata Chhabra, Manmeet Kaur Gill |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty Tuberculosis Pleural tuberculosis Clinical Biochemistry lcsh:Medicine is6110 sequence Microbiology law.invention Mycobacterium tuberculosis Adenosine deaminase law medicine Polymerase chain reaction mycobacterium tuberculosis nested polymerase chain reaction biology business.industry lcsh:R General Medicine Gold standard (test) biology.organism_classification medicine.disease Staining pleural tuberculosis biology.protein Original Article business Nested polymerase chain reaction |
| Zdroj: | Journal of Clinical and Diagnostic Research, Vol 11, Iss 11, Pp 2456-2458 (2013) |
| Popis: | Background: Early diagnosis of tuberculosis is important in its control. The conventional techniques like smear microscopy and culture suffer from low sensitivity for diagnosis of extra-pulmonary tuberculosis like Pleural Tuberculosis (PTB) due to paucibacillary nature of the fluid. Polymerase Chain Reaction (PCR) is presently seen as a promising alternative to conventional techniques. In this study we have evaluated IS6110 sequence based nested PCR (nPCR) for the detection of Mycobacterium tuberculosis (MTB) DNA directly from clinical samples. The results of PCR were compared with the results of conventional methods like smear, culture and Adenosine Deaminase (ADA) activity. Material and Methods: A total of 50 pleural fluid samples from the patients with history suggestive of tuberculosis were taken. All the samples were processed for Ziehl-Neelsan (ZN) staining for Acid Fast Bacilli (AFB), culture ADA activity and PCR with primers targeting 123bp fragment of IS6110 of MTB complex. Results: A significant difference was seen in the sensitivities of conventional methods and PCR (p |
| Databáze: | OpenAIRE |
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