New Insights into the Effects of Chronic Kidney Failure and Dialysate Exposure on the Peritoneum
| Autor: | Dirk G. Struijk, Dirk R. de Waart, Carmen A. Vlahu, Jan Aten, Vincent Everts, Marijke de Graaff, Raymond T. Krediet, Henk A. van Veen |
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| Rok vydání: | 2016 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment 030232 urology & nephrology Urology Renal function 030204 cardiovascular system & hematology Kidney Function Tests Nephrectomy Epithelium Statistics Nonparametric Peritoneal dialysis Random Allocation 03 medical and health sciences Hydroxyproline chemistry.chemical_compound 0302 clinical medicine Peritoneum Risk Factors Fibrosis Dialysis Solutions medicine Animals Rats Wistar Dialysis Analysis of Variance Chi-Square Distribution business.industry Biopsy Needle Peritoneal Fibrosis Original Articles General Medicine medicine.disease Immunohistochemistry Rats Disease Models Animal medicine.anatomical_structure chemistry Nephrology Kidney Failure Chronic Lymph business Peritoneal Dialysis |
| Zdroj: | Peritoneal Dialysis International: Journal of the International Society for Peritoneal Dialysis. 36:614-622 |
| ISSN: | 1718-4304 0896-8608 |
| Popis: | IntroductionChronic uremia and the exposure to dialysis solutions during peritoneal dialysis (PD) induce peritoneal alterations. Using a long-term peritoneal exposure model, we compared the effects of chronic kidney failure (CKD) itself and exposure to either a ‘conventional’ or a ‘biocompatible’ dialysis solution on peritoneal morphology and function.MethodsWistar rats (Harlan, Zeist, the Netherlands) were grouped into: normal kidney function (NKF), CKD induced by 70% nephrectomy, CKD receiving daily peritoneal infusions with 3.86% glucose Dianeal (CKDD), or Physioneal (both solutions from Baxter Healthcare, Castlebar, Ireland) (CKDP). At 16 weeks, a peritoneal function test was performed, and histology, ultrastructure, and hydroxyproline content of peritoneal tissue were assessed.ResultsComparing CKD with NKF, peritoneal transport rates were higher, mesothelial cells (MC) displayed increased number of microvilli, blood and lymph vasculature expanded, vascular basal lamina appeared thicker, with limited areas of duplication, and fibrosis had developed. All alterations, except lymphangiogenesis, were enhanced by exposure to both dialysis fluids. Distinct MC alterations were observed in CKDD and CKDP, the latter displaying prominent basolateral protrusions. In addition, CKDP was associated with a trend towards less fibrosis compared to CKDD.ConclusionsChronic kidney failure itself induced peritoneal alterations, which were in part augmented by exposure to glucose-based dialysis solutions. Overall, the conventional and biocompatible solutions had similar long-term effects on the peritoneum. Importantly, the latter may attenuate the development of fibrosis. |
| Databáze: | OpenAIRE |
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