Neuroprotective fragment C of tetanus toxin modulates IL-6 in an ALS mouse model
| Autor: | Rosario Osta, Laura Moreno-Martínez, Pilar Zaragoza, José Aguilera, Miriam de la Torre, Ana C. Calvo, María Jesús Muñoz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
amyotrophic lateral sclerosis Inflammasomes Health Toxicology and Mutagenesis Anti-Inflammatory Agents lcsh:Medicine Nod Pharmacology Toxicology Pyrin domain Superoxide Dismutase-1 0302 clinical medicine Macrophage inflammatory protein 0303 health sciences biology Chemistry Communication Caspase 1 Interleukin Neuroprotective Agents Spinal Cord Female Inflammation Mediators medicine.symptom TTC Down-Regulation Mice Transgenic Inflammation Neuroprotection SOD1G93A mouse model 03 medical and health sciences Tetanus Toxin NLR Family Pyrin Domain-Containing 3 Protein medicine Animals cardiovascular diseases Muscle Skeletal Interleukin 6 Neuroinflammation 030304 developmental biology Interleukin-6 lcsh:R Amyotrophic lateral sclerosis Peptide Fragments Mice Inbred C57BL Disease Models Animal inflammation biology.protein 030217 neurology & neurosurgery |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Zaguán: Repositorio Digital de la Universidad de Zaragoza Universidad de Zaragoza Toxins Toxins, Vol 12, Iss 330, p 330 (2020) Zaguán. Repositorio Digital de la Universidad de Zaragoza instname |
| Popis: | Neuroinflammation plays a significant role in amyotrophic lateral sclerosis (ALS) pathology, leading to the development of therapies targeting inflammation in recent years. Our group has studied the tetanus toxin C-terminal fragment (TTC) as a therapeutic molecule, showing neuroprotective properties in the SOD1G93A mouse model. However, it is unknown whether TTC could have some effect on inflammation. The objective of this study was to assess the effect of TTC on the regulation of inflammatory mediators to elucidate its potential role in modulating inflammation occurring in ALS. After TTC treatment in SOD1G93A mice, levels of eotaxin-1, interleukin (IL)-2, IL-6 and macrophage inflammatory protein (MIP)-1 alpha (α) and galectin-1 were analyzed by immunoassays in plasma samples, whilst protein expression of caspase-1, IL-1β, IL-6 and NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) was measured in the spinal cord, extensor digitorum longus (EDL) muscle and soleus (SOL) muscle. The results showed reduced levels of IL-6 in spinal cord, EDL and SOL in treated SOD1G93A mice. In addition, TTC showed a different role in the modulation of NLRP3 and caspase-1 depending on the tissue analyzed. In conclusion, our results suggest that TTC could have a potential anti-inflammatory effect by reducing IL-6 levels in tissues drastically affected by the disease. However, further research is needed to study more in depth the anti-inflammatory effect of TTC in ALS. |
| Databáze: | OpenAIRE |
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