Accurate and Rapid Identification of the Burkholderia pseudomallei Near-Neighbour, Burkholderia ubonensis, Using Real-Time PCR

Autor: Paul Keim, Jessica R. Webb, Derek S. Sarovich, Apichai Tuanyok, Vanessa Theobald, Joseph D. Busch, Jeffrey T. Foster, Erin P. Price, Bart J. Currie, Carina M. Hall, David M. Wagner, Mirjam Kaestli, James M. Cook, Talima Pearson, Meagan L. Seymour, Jennifer L. Ginther, Mark Mayo
Rok vydání: 2013
Předmět:
Bacterial Diseases
Burkholderia pseudomallei
Melioidosis
Burkholderia cenocepacia
Applied Microbiology
lcsh:Medicine
law.invention
law
Genome Databases
Gram Negative
Genome Sequencing
lcsh:Science
Soil Microbiology
Polymerase chain reaction
0303 health sciences
Multidisciplinary
biology
Biodiversity
Genomics
Bacterial Pathogens
Phenotype
Infectious Diseases
Medicine
Water Microbiology
Research Article
Burkholderia ubonensis
Burkholderia
Sequence Databases
Bacterial genome size
Real-Time Polymerase Chain Reaction
Microbiology
Microbial Ecology
Burkholderia Cepacia Complex
03 medical and health sciences
Genome Analysis Tools
Microbial Control
medicine
Biology
Microbial Pathogens
030304 developmental biology
030306 microbiology
lcsh:R
Sequence Analysis
DNA
Comparative Genomics
bacterial infections and mycoses
biology.organism_classification
medicine.disease
Burkholderia cepacia complex
bacteria
lcsh:Q
Genome
Bacterial
Zdroj: PLoS ONE, Vol 8, Iss 8, p e71647 (2013)
PLoS ONE
ISSN: 1932-6203
Popis: Burkholderia ubonensis is an environmental bacterium belonging to the Burkholderia cepacia complex (Bcc), a group of genetically related organisms that are associated with opportunistic but generally nonfatal infections in healthy individuals. In contrast, the near-neighbour species Burkholderia pseudomallei causes melioidosis, a disease that can be fatal in up to 95% of cases if left untreated. B. ubonensis is frequently misidentified as B. pseudomallei from soil samples using selective culturing on Ashdown’s medium, reflecting both the shared environmental niche and morphological similarities of these species. Additionally, B. ubonensis shows potential as an important biocontrol agent in B. pseudomallei-endemic regions as certain strains possess antagonistic properties towards B. pseudomallei. Current methods for characterising B. ubonensis are laborious, time-consuming and costly, and as such this bacterium remains poorly studied. The aim of our study was to develop a rapid and inexpensive real-time PCR-based assay specific for B. ubonensis. We demonstrate that a novel B. ubonensis-specific assay, Bu550, accurately differentiates B. ubonensis from B. pseudomallei and other species that grow on selective Ashdown’s agar. We anticipate that Bu550 will catalyse research on B. ubonensis by enabling rapid identification of this organism from Ashdown’s-positive colonies that are not B. pseudomallei.
Databáze: OpenAIRE
Externí odkaz: