Functional Co-assembly among Subunits of Cyclic-Nucleotide-Activated, Nonselective Cation Channels, and Across Species from Nematode to Human
| Autor: | Tsung-Yu Chen, J. T. Finn, Randall R. Reed, King Wai Yau, J. E. Schroeder, D. Krautwurst |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Nematoda
Macromolecular Substances Protein subunit Biophysics Kidney Transfection Ion Channels Cell Line 03 medical and health sciences 0302 clinical medicine Species Specificity Retinal Rod Photoreceptor Cells Cyclic AMP Animals Humans Homomeric Caenorhabditis elegans Cyclic GMP Vision Ocular Ion channel 030304 developmental biology Genetics 0303 health sciences biology HEK 293 cells biology.organism_classification Recombinant Proteins Rats Cell biology Kinetics Drosophila melanogaster Vertebrates Retinal Cone Photoreceptor Cells Photoreceptor Cells Invertebrate 030217 neurology & neurosurgery Research Article Visual phototransduction |
| Zdroj: | Biophysical Journal. 74:1333-1345 |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/s0006-3495(98)77846-4 |
| Popis: | Cyclic-nucleotide-activated, nonselective cation channels have a central role in sensory transduction. They are most likely tetramers, composed of two subunits ( α and β or 1 and 2), with the former, but not the latter, being able to form homomeric cyclic-nucleotide-activated channels. Identified members of this channel family now include, in vertebrates, the rod and cone channels mediating visual transduction and the channel mediating olfactory transduction, each apparently with distinct α - and β -subunits. Homologous channels have also been identified in Drosophila melanogaster and Caenorhabditis elegans . By co-expressing any combination of two α -subunits, or α - and β -subunits, of this channel family in HEK 293 cells, we have found that they can all co-assemble functionally with each other, including those from fly and nematode. This finding suggests that the subunit members so far identified form a remarkably homogeneous and conserved group, functionally and evolutionarily, with no subfamilies yet identified. The ability to cross-assemble allows these subunits to potentially generate a diversity of heteromeric channels, each with properties specifically suited to a particular cellular function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|