Relationship of Molecular Size of rIn:rCn (Poly I:C) to Induction of Interferon and Host Resistance
| Autor: | Nemes Mm, Field Ak, Alfred A. Tytell, Maurice R. Hilleman, Lampson Gp |
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| Rok vydání: | 1970 |
| Předmět: |
Optics and Photonics
Chemical Phenomena Thermal transition Pneumonia Viral Polynucleotides Cytosine Nucleotides Vesicular stomatitis Indiana virus General Biochemistry Genetics and Molecular Biology Mice Ribonucleases Molecular size Interferon Culture Techniques medicine Animals Ultrasonics I²C Chromatography Host resistance biology Viscosity Chemistry Hyperchromicity Nucleosides Molecular biology Molecular Weight Biochemistry Polynucleotide biology.protein Pancreatic ribonuclease Interferons Rabbits medicine.drug |
| Zdroj: | Experimental Biology and Medicine. 135:911-916 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-135-35169 |
| Popis: | SummaryPreparations of high molecular weight rIn:rCn were exposed to sonic radiation. A decrease in the average molecular weight of the polynucleotide complexes after treatment was accompanied by an exponential decrease in viscosity of the preparation, an increase in the sensitivity of the polynucleotide complex to degradation by pancreatic ribonuclease, a decrease in the thermal transition midpoint, and a decrease in the hyperchromicity observed during thermal transition.The lower molecular weight rIn:rCn complexes were tested for their capacity to induce interferon in rabbits and resistance against VSV infection in cell culture and PVM infection in mice. Sonic-treated preparations of rIn:rCn reduced to an average molecular weight of approximately 4.6 × 105 were markedly reduced in capacity to protect mice against PVM infection, compared with untreated rIn:rCn preparations containing complexes having an average molecular weight of approximately 7.8 × 106. The capacity of sonic-treated rIn:rCn to induce p... |
| Databáze: | OpenAIRE |
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