Construction of a 10,000-Marker Ultradense Genetic Recombination Map of Potato: Providing a Framework for Accelerated Gene Isolation and a Genomewide Physical Map
| Autor: | Glenn J. Bryan, Véronique Lefebvre, Walter De Jong, Herman J. van Eck, Edwige Isidore, Richard G. F. Visser, Imanol Barrena, Enrique Ritter, Robbie Waugh, Bernard Caromel, Hans van Os, Joke van Vliet, Erin Bakker, Jeroen Rouppe van der Voort, bilal ahmad ahmad ghareeb, Françoise Rousselle-Bourgeois, Jaap Bakker, Sandra Andrzejewski, Dan Milbourne, Paul van Koert |
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| Přispěvatelé: | Laboratory of plant breeding, Wageningen University and Research [Wageningen] (WUR), Génétique et Amélioration des Fruits et Légumes (GAFL), Institut National de la Recherche Agronomique (INRA), Laboratory of Nematology, Instituto Vasco de Investigación y Desarrollo Agrario [Derio] (NEIKER), Scottish Crop Research Institute |
| Rok vydání: | 2006 |
| Předmět: |
0106 biological sciences
Restriction Mapping solanum-tuberosum tomato 01 natural sciences Genetic recombination Laboratorium voor Plantenveredeling ComputingMilieux_MISCELLANEOUS Recombination Genetic 2. Zero hunger Genetics density 0303 health sciences education.field_of_study Contig EPS-4 Chromosome Mapping food and beverages Chiasma Meiosis sequence-tagged sites Genome Plant Genetic Markers Heterozygote Quantitative Trait Loci Population CARTOGRAPHIE GENETIQUE Investigations Biology Sequence-tagged site 03 medical and health sciences Centimorgan aflp markers Genetic linkage education Laboratorium voor Nematologie resistance-gene Solanum tuberosum 030304 developmental biology [SDV.GEN]Life Sciences [q-bio]/Genetics populations linkage map Diploidy Plant Breeding Genetic marker mapping strategy quantitative trait loci Laboratory of Nematology 010606 plant biology & botany |
| Zdroj: | Genetics Genetics, Genetics Society of America, 2006, 173 (2), pp.1075-1087. ⟨10.1534/genetics.106.055871⟩ Genetics 173 (2006) 2 Genetics, 173(2), 1075-1087 |
| ISSN: | 1943-2631 0016-6731 |
| Popis: | An ultradense genetic linkage map with >10,000 AFLP loci was constructed from a heterozygous diploid potato population. To our knowledge, this is the densest meiotic recombination map ever constructed. A fast marker-ordering algorithm was used, based on the minimization of the total number of recombination events within a given marker order in combination with genotyping error-detection software. This resulted in “skeleton bin maps,” which can be viewed as the most parsimonious marker order. The unit of distance is not expressed in centimorgans but in “bins.” A bin is a position on the genetic map with a unique segregation pattern that is separated from adjacent bins by a single recombination event. Putative centromeres were identified by a strong clustering of markers, probably due to cold spots for recombination. Conversely, recombination hot spots resulted in large intervals of up to 15 cM without markers. The current level of marker saturation suggests that marker density is proportional to physical distance and independent of recombination frequency. Most chromatids (92%) recombined once or never, suggesting strong chiasma interference. Absolute chiasma interference within a chromosome arm could not be demonstrated. Two examples of contig construction and map-based cloning have demonstrated that the marker spacing was in accordance with the expected physical distance: approximately one marker per BAC length. Currently, the markers are used for genetic anchoring of a physical map of potato to deliver a sequence-ready minimal tiling path of BAC contigs of specific chromosomal regions for the potato genome sequencing consortium (http://www.potatogenome.net). |
| Databáze: | OpenAIRE |
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