Cyclophilin B enhances HIV-1 infection
Autor: | Michael Belshan, Christian J. Madson, Jason DeBoer |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
CypB Nuclear import T-Lymphocytes Amino Acid Motifs HIV Infections Biology Virus-host interactions Article Cyclophilins 03 medical and health sciences Virology medicine Humans Secretion Cyclophilin b Cell Nucleus Group-specific antigen Molecular biology CYCLOPHILIN B Cell nucleus 030104 developmental biology medicine.anatomical_structure Chaperone (protein) Host-Pathogen Interactions HIV-1 biology.protein RNA Viral Nuclear transport Intracellular Nuclear localization sequence |
Zdroj: | Virology. 489:282-291 |
ISSN: | 0042-6822 |
Popis: | Cyclophilin B (CypB) is a member of the immunophilin family and intracellular chaperone. It predominantly localizes to the ER, but also contains a nuclear localization signal and is secreted from cells. CypB has been shown to interact with the Gag protein of human immunodeficiency type 1 (HIV-1). Several proteomic and genetic studies identified it as a potential factor involved in HIV replication. Herein, we show that over-expression of CypB enhances HIV infection by increasing nuclear import of viral DNA. This enhancement was unaffected by cyclosporine treatment and requires the N-terminus of the protein. The N-terminus contains an ER leader sequence, putative nuclear localization signal, and is required for secretion. Deletion of the N-terminus resulted in mislocalization from the ER and suppression of HIV infection. Passive transfer experiments showed that secreted CypB did not impact HIV infection. Combined, these experiments show that intracellular CypB modulates a pathway of HIV nuclear import. |
Databáze: | OpenAIRE |
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