Synthetic menthyl α/β-(1→6)-diglucopyranosides-induced cell death in human leukemia cells is dependent on caspases
| Autor: | Sara Tejera, Francisco Estévez, Carlos Mayato, Sara Estévez, María Teresa Marrero, José Quintana, Jesús T. Vázquez, Rosa L. Dorta |
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| Rok vydání: | 2012 |
| Předmět: |
Programmed cell death
Poly ADP ribose polymerase Clinical Biochemistry Pharmaceutical Science Apoptosis HL-60 Cells Disaccharides Biochemistry Amino Acid Chloromethyl Ketones Cell Line Tumor Drug Discovery Cytotoxic T cell Humans Cytotoxicity Molecular Biology Caspase bcl-2-Associated X Protein Leukemia biology Chemistry Cytochrome c Organic Chemistry Intrinsic apoptosis Cytochromes c Molecular biology Cell biology Caspases biology.protein Molecular Medicine Reactive Oxygen Species |
| Zdroj: | Bioorganicmedicinal chemistry letters. 22(11) |
| ISSN: | 1464-3405 |
| Popis: | A series of alkyl α/β-(1→6)-diglucopyranosides 1−12 were synthesized and assessed for cytotoxicity against HL-60, U937, Molt-3 and MCF-7 cancer cell lines. The menthyl derivatives displayed strong cytotoxic properties showing IC 50 values between 6 and 16 μM. Furthermore, we demonstrated that the selected synthetic (+)-menthyl β-(1→6)-diglucopyranoside 5 induces apoptotic cell death in human leukemia cells through a mechanism that involves activation of multiple caspases. Cell death was completely prevented by the non-specific caspase inhibitor z-VAD-fmk and found to be associated with the release of cytochrome c , an increase in the expression of Bax levels and a decrease in the generation of reactive oxygen species. |
| Databáze: | OpenAIRE |
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