Sodium deoxycholate inhibits chick duodenal calcium absorption through oxidative stress and apoptosis
| Autor: | A.M. Marchionatti, Nori Tolosa de Talamoni, Gabriela Díaz de Barboza, María E. Peralta López, M.A. Rivoira, V. Centeno |
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| Rok vydání: | 2012 |
| Předmět: |
Calbindins
Duodenum Physiology ATPase Gene Expression Apoptosis Mitochondrion medicine.disease_cause Biochemistry Antioxidants Sodium-Calcium Exchanger Avian Proteins Plasma Membrane Calcium-Transporting ATPases chemistry.chemical_compound S100 Calcium Binding Protein G medicine Animals Intestinal Mucosa Transcellular Molecular Biology Calcium metabolism biology Chemistry Cytochrome c Cytochromes c Glutathione Mitochondria Oxidative Stress Enterocytes Intestinal Absorption biology.protein Calcium Quercetin Reactive Oxygen Species Chickens Oxidative stress Deoxycholic Acid |
| Zdroj: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 162:397-405 |
| ISSN: | 1095-6433 |
| Popis: | High concentrations of sodium deoxycholate (NaDOC) produce toxic effects. This study explores the effect of a single high concentration of NaDOC on the intestinal Ca 2 + absorption and the underlying mechanisms. Chicks were divided into two groups: 1) controls and 2) treated with different concentrations of NaDOC in the duodenal loop for variable times. Intestinal Ca 2 + absorption was measured as well as the gene and protein expressions of molecules involved in the Ca 2 + transcellular pathway. NaDOC inhibited the intestinal Ca 2 + absorption, which was concentration dependent. Ca 2 + -ATPase mRNA decreased by the bile salt and the same occurred with the protein expression of Ca 2 + -ATPase, calbindin D 28 k and Na + /Ca 2 + exchanger. NaDOC produced oxidative stress as judged by ROS generation, mitochondrial swelling and glutathione depletion. Furthermore, the antioxidant quercetin blocked the inhibitory effect of NaDOC on the intestinal Ca 2 + absorption. Apoptosis was also triggered by the bile salt, as indicated by the TUNEL staining and the cytochrome c release from the mitochondria. As a compensatory mechanism, enzyme activities of the antioxidant system were all increased. In conclusion, a single high concentration of NaDOC inhibits intestinal Ca 2 + absorption through downregulation of proteins involved in the transcellular pathway, as a consequence of oxidative stress and mitochondria mediated apoptosis. |
| Databáze: | OpenAIRE |
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