Prenatal Ethanol Exposure Alters the Expression of Intestinal Hydrolase mRNAs in Newborn Rats
| Autor: | Hans A. Büller, M. Dolores López-Tejero, Edmond H.H.M. Rings, Stephen D. Krasinski, Richard J. Grand, Gemma Estrada |
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| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Amniotic fluid Enterocyte medicine.medical_treatment Medicine (miscellaneous) Cell Count Biology Toxicology Pregnancy Internal medicine medicine Animals Lactase-Phlorizin Hydrolase RNA Messenger In Situ Hybridization Lactase-phlorizin hydrolase Fetus Lactase Alkaline Phosphatase Amniotic Fluid Immunohistochemistry Small intestine Rats Intestines Disease Models Animal Psychiatry and Mental health Endocrinology medicine.anatomical_structure Animals Newborn Intestinal Absorption Fetal Alcohol Spectrum Disorders In utero Alkaline phosphatase Female |
| Zdroj: | Alcoholism: Clinical and Experimental Research. 20:1662-1668 |
| ISSN: | 0145-6008 |
| DOI: | 10.1111/j.1530-0277.1996.tb01713.x |
| Popis: | To gain insight into the postnatal growth delay induced by ethanol in utero, we characterized functional impairments of the small intestine of neonatal rats prenatally exposed to ethanol using a well-described model of gestational alcoholism (25% ethanol w/v in the drinking water). Expression of the intestinal enzymes-lactase-phlorizin hydrolase (LPH) and intestinal alkaline phosphatase (IAP)-that are critical for enteral nutrition of neonates was studied. Characteristic patterns of LPH and IAP expression along the proximal-distal (horizontal) and crypt-villus (vertical) axes of the small intestine, as well as the intracellular localization of LPH and IAP mRNAs and immunoreactive proteins within absorptive enterocytes, were not altered by prenatal exposure to ethanol. However, a 10- to 15-fold increase in the number of LPH and IAP mRNA molecules per absorptive enterocyte was found throughout the intestine of ethanol-exposed neonates, compared with controls, whereas lactase and alkaline phosphatase activities per enterocyte remained unchanged. These findings suggest that ethanol in utero alters the mRNA abundance of epithelial enzymes in newborn rat small intestine. Changes in mRNA abundance could be an important aspect of enterocyte adaptation to high ethanol concentrations in gastrointestinal amniotic fluid of ethanol-exposed fetuses. |
| Databáze: | OpenAIRE |
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