Spin-labeling of Dexamethasone
| Autor: | Martina C. Meinke, Silke B. Lohan, Reinhold Zimmer, Michael Unbehauen, Karolina A Walker, Siavash Saeidpour, Rainer Haag |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
ex vivo imaging
X band epr Chemistry electron spin relaxation 02 engineering and technology Site-directed spin labeling 021001 nanoscience & nanotechnology 030226 pharmacology & pharmacy X-band EPR law.invention 03 medical and health sciences 0302 clinical medicine Nuclear magnetic resonance 500 Natural sciences and mathematics::530 Physics::530 Physics law Temporal resolution Physical and Theoretical Chemistry 0210 nano-technology Electron paramagnetic resonance labeling |
| DOI: | 10.17169/refubium-2281 |
| Popis: | Spin-labeling active compounds is a convenient way to prepare them for EPR spectroscopy with minimal alteration of the target molecule. In this study we present the labeling reaction of dexamethasone (Dx) with either TEMPO (2,2,6,6-tetramethyl-1-piperidinyloxy) or PCA (3-(carboxy)-2,2,5,5-tetramethyl-1-pyrrolidinyloxy) with high yields. According to NMR data, both labels are attached at the primary hydroxy group of the steroid. In subsequent spin-stability measurements both compounds were applied onto HaCaT cells. When the signal of Dx-TEMPO decreased below the detection limit within 3 h, the signal of Dx-PCA remained stable for the same period of time. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|