A C. elegans Homolog of Huntingtin-Associated Protein 1 is Expressed in Chemosensory Neurons and in a Number of Other Somatic Cell Types
| Autor: | Erin Manning, Guy M. Benian, Kim M. Gernert, Walter W. Walthall, Kristina B. Mercer, Sarah M. Szlam, Claire-Anne Gutekunst |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cell type
Huntingtin Somatic cell Green Fluorescent Proteins Molecular Sequence Data Nerve Tissue Proteins Biology Article Green fluorescent protein Animals Genetically Modified Cellular and Molecular Neuroscience RNA interference Animals Amino Acid Sequence Cloning Molecular Caenorhabditis elegans Caenorhabditis elegans Proteins Promoter Regions Genetic Base Sequence Huntingtin-associated protein 1 fungi Amphid General Medicine biology.organism_classification Molecular biology Chemoreceptor Cells nervous system biology.protein RNA Interference |
| Zdroj: | Journal of Molecular Neuroscience. 37:37-49 |
| ISSN: | 1559-1166 0895-8696 |
| Popis: | Huntingtin Associated Protein 1 (HAP1) is a binding partner for huntingtin, the protein responsible for Huntington’s Disease. In mammals, HAP1 is mostly found in brain where it is expressed in neurons. Although several functions have been proposed for HAP1, its role has not yet been clearly established. Here we report on the identification of a HAP1 C.elegans homolog called T27A3.1. T27A3.1 shows conservation with rat and human HAP1 as well as with Milton, a Drosophila HAP1 homolog. To determine the cellular expression of T27A3.1 (multiple isoforms; a-e), we generated several transgenic worm lines expressing a fluorescent reporter protein (GFP and DsRed2) or full length T27A3.1a/c isoforms fused to GFP under the control of the promoter for T27A3.1. We have found that T27A3.1 is expressed in many cell types including a subset of chemosensory neurons in the head and tail. These include the amphid chemosensory neurons ASKL and R, ASIL and R, ADFL and ASEL; the phasmid neurons PHBL and R; and the CAN neurons which are required for worm survival. Furthermore, we show that the subcellular localization of T27A3.1a/c resemble that of mammalian HAP1 and that T27A3.1a/c localize to stigmoid body like structures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink