Antigen Mimicry by Anti-idiotypic Antibodies: Study of Interactions between Complementary Surfaces in Macromolecules
| Autor: | Ronald L. Somerville, Weiping Yang |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Operator (biology)
Enzyme-Linked Immunosorbent Assay General Biochemistry Genetics and Molecular Biology Antigen-Antibody Reactions chemistry.chemical_compound Bacterial Proteins Antigen Animals New zealand white Antigens Molecular Biology Immune repertoire biology Tryptophan DNA Molecular biology Antibodies Anti-Idiotypic Repressor Proteins Biochemistry chemistry biology.protein Anti-idiotypic antibodies Rabbits Antibody Macromolecule |
| Zdroj: | Methods. 19:322-329 |
| ISSN: | 1046-2023 |
| Popis: | Anti-idiotypic antibodies were obtained from New Zealand White rabbits injected with affinity-purified rabbit anti-TrpR antibodies. In gel mobility shift studies, such immunoglobulin preparations were shown to contain one or more species able to form specific complexes with DNA molecules bearing a trp operator. In competitive ELISA assays, the binding of anti-idiotypic antibodies to operator-bearing DNA was reversed by TrpR. The demonstration that the immune repertoire contains information for operator-specific DNA-binding proteins may be relevant to the etiology of certain autoimmune diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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