MicroRNA‑302a inhibits cell proliferation and invasion, and induces cell apoptosis in hepatocellular carcinoma by directly targeting VEGFA

Autor: Chen Wang, Wen-Zhang Zha, Yong-Hua Xu, Hui-Min Ding, Cheng-Lin Qin, Ren-Gen Fan
Rok vydání: 2016
Předmět:
0301 basic medicine
Oncology
Male
Vascular Endothelial Growth Factor A
Cancer Research
Cell
Biochemistry
0302 clinical medicine
Cell Movement
RNA
Small Interfering
Liver Neoplasms
Hep G2 Cells
Cell cycle
Middle Aged
Gene Expression Regulation
Neoplastic
Vascular endothelial growth factor A
medicine.anatomical_structure
030220 oncology & carcinogenesis
Lymphatic Metastasis
Molecular Medicine
Female
A431 cells
Signal Transduction
Adult
medicine.medical_specialty
Carcinoma
Hepatocellular
Biology
03 medical and health sciences
Internal medicine
Cell Line
Tumor
microRNA
Genetics
medicine
Humans
neoplasms
Molecular Biology
Aged
Cell Proliferation
Neoplasm Staging
Oligoribonucleotides
Oncogene
Cell growth
Cancer
Antagomirs
medicine.disease
digestive system diseases
MicroRNAs
030104 developmental biology
Neoplasm Grading
Zdroj: Molecular medicine reports. 16(5)
ISSN: 1791-3004
Popis: Hepatocellular carcinoma (HCC) is the fifth most common cancer and the third most common cause of cancer‑related mortality worldwide. An increasing number of studies have demonstrated that microRNAs may be used as diagnostic, therapeutic and prognostic targets for human cancers, including HCC. The present study aimed to evaluate microRNA (miR)‑302a expression and function in HCC, and its underlying mechanisms. The results revealed that miR‑302a was expressed at low levels in HCC tissues and cell lines. Reduced miR‑302a expression was correlated with tumor‑node‑metastasis stage and lymph node metastasis in patients with HCC. Additionally, overexpression of miR‑302a reduced cell proliferation and invasion, and induced apoptosis in HCC cells. Vascular endothelial growth factor A (VEGFA) was demonstrated to be a direct target gene of miR‑302a. VEGFA was highly expressed in HCC tissues and inversely correlated with miR‑302a expression. Knockdown of VEGFA expression led to reduced HCC cell proliferation and invasion, and increased apoptosis rates, similar to miR‑302a overexpression, which suggested that VEGFA may be a functional downstream target of miR‑302a in HCC. These data suggested that this newly identified miR‑302a/VEGFA axis may be involved in HCC formation and progression. The present results also provide novel potential targets for the treatments of patients with HCC.
Databáze: OpenAIRE
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