Direct observation of dynamic mechanical regulation of DNA condensation by environmental stimuli
| Autor: | Amy Lee, Jason M. Hustedt, Adam Paul Karcz, Lucas Tricoli, Ryan Akman, Szu-Ting Chou, Tai Zheng, Joonil Seog, A. James Mixson, Qixin Leng, Jason D. Kahn, Sarah Sucayan, Sara Kwon |
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| Rok vydání: | 2014 |
| Předmět: |
Optical Tweezers
Cations Divalent Molecular Sequence Data Nanotechnology Gene delivery DNA condensation Catalysis Article chemistry.chemical_compound Histidine Polylysine Amino Acid Sequence Chemistry Lysine Relaxation (NMR) Direct observation Gene Transfer Techniques General Medicine General Chemistry Transfection DNA Zinc Optical tweezers Biophysics Nucleic acid Nucleic Acid Conformation Peptides |
| Zdroj: | Angewandte Chemie (International ed. in English). 53(40) |
| ISSN: | 1521-3773 |
| Popis: | Gene delivery is a promising way to treat hereditary diseases and cancer; however, there is little understanding of DNA:carrier complex mechanical properties, which may be critical for the protection and release of nucleic acids. We applied optical tweezers to directly measure single-molecule mechanical properties of DNA condensed using 19-mer poly-L-lysine (PLL) or branched histidine–lysine (HK) peptides. Force–extension profiles indicate that both carriers condense DNA actively, showing force plateaus during stretching and relaxation cycles. As the environment such as carrier concentration, pH, and the presence of zinc ions changes, DNA:HK complexes showed dynamically regulated mechanical properties at multiple force levels. The fundamental knowledge from this study can be applied to design a mechanically tailored complex which may enhance transfection efficiency by controlling the stability of the complex temporally and spatially. |
| Databáze: | OpenAIRE |
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